We narrowed to 2,200 results for: dcas9

1. iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP

   Plasmid

   #204722

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGB3 omega1 P35S:MS2:VPR:Tnos - P35S:dCas9:EDLL:Tnos (GB2085)

   Plasmid

   #160645

   Purpose

   Module for the expression of Ms2 protein fused to VPR and dCas9 fused to EDLL

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35s-Ms2:VPR-Tnos-35s-dCas9:EDLL-Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsmBI sites removed

   Promoter

   35S x2

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

   Plasmid

   #101104

   Purpose

   Encodes dCas9(N) fused to VEGFR2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   VEGFR2-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

   Plasmid

   #210710

   Purpose

   This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   M11

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin

   Plasmid

   #187954

   Purpose

   FKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   FKBP12_F36V-SpdCas9-hHDAC4-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

   Plasmid

   #192665

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64, ZsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSD_2XNLS-SpCas9WT-NLS-3XHA-2XNLS-NmdCas9-NLS

   Plasmid

   #107327

   Purpose

   Expresses SpCas9 fused to dNmCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-NmCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 is fused to nuclease dead NmCas9 (D16A, D5…

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-BD10-SAS620-3’dCas9-VPR-synpA

   Plasmid

   #216323

   Purpose

   Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Cas9-VPR + splice acceptor site
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-3xsgMyo7b-CMV-5’dCas9-SDS-BD10-SV40pA

   Plasmid

   #216322

   Purpose

   Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Cas9-VPR + splice donor site + gRNAs targeting the murine Myo7b locus for transactivation
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

    Plasmid

    #112214

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3A-L-dcas9 (DNMT3L, DNMT3A Human)
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mutation

    D10A,H840A,N863A

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101102

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    TMt-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187945

    Purpose

    dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    Synthetic Biology; Piggybac

    Tags

    NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1

    Plasmid

    #121831

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/LSD1 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

    Plasmid

    #112215

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3A, DNMT3L Human)
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mutation

    D10A,H840A,N863A,C710S

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

    Plasmid

    #192666

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Insert

    dCas9-VP64, MS2-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101101

    Purpose

    Encodes dCas9(N) fused to transmembrane tether

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    TMt-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid

    #121833

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/Dnmt3a-3L (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101107

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101108

    Purpose

    Encodes dCas9(N) fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

    Plasmid

    #170808

    Purpose

    PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Inserts

    GID1-VPR-IRES-HygR
    GAI-tagBFP-Sa_dCas9 (GAI Mustard Weed, S. aureus, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous mutation in PYL1 to remove XhoI site (…

    Promoter

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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