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pSB3C5-proA-B0032-E0051

Plasmid

#107244

Purpose

Expresses lacZa-GFP fusion from constitutive proA promoter

Depositor

Joseph Davis, Robert Sauer

Publication

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProA-RBS(B0032)-Gemini (E0051)

Promoter

proA

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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T7-phiC31-nos3'UTR

Plasmid

#182192

Purpose

in vitro transcription of phiC31 integrase mRNA

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

phiC31 integrase

Use

In vitro transcription

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8

Plasmid

#121111

Purpose

conditional expression

Depositor

Emily Dykhuizen

Publication

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 (CBX8 Human)

Use

Lentiviral

Tags

V5

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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VVVVV

Plasmid

#29426

Depositor

Steven Vogel

Publication

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-5-Venus-5-Venus-5-Venus-5-Venus

Use

Anisotropy and brightness standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMTCbLCVA::CH42

Plasmid

#22786

Depositor

Niki Robertson

Publication

Turnage et al Plant J. 2002 Apr . 30(1):107-14.

Insert

A DNA containing CHLI fragment

Use

RNAi; Arabidopsis vigs vector

Mutation

N/A

Available since

July 8, 2010

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Violet

Plasmid

#160447

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Violet

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Gap43

Plasmid

#74336

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

growth associated protein 43 (Gap43 Mouse)

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Publication

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Expression

Bacterial

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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p177bp-LacOx162-Hyg

Plasmid

#210215

Purpose

Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Publication

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

162xLacO repeats derived from: PMID: 12864855.

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBW103ParsR-Amp30C

Plasmid

#78638

Purpose

pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Publication

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

30hrpR-30hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pI7-ISH

Plasmid

#15527

Depositor

Peter Mombaerts

Publication

Fuss et al Cell. 2007 Jul 27. 130(2):373-84.

Insert

I7 (Or6a2 Mouse)

Use

For riboprobe, in situ hybridization

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pBW300ParsR-Amp32T

Plasmid

#78652

Purpose

pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Publication

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-

Use

Synthetic Biology

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pDelila (GB0079)

Plasmid

#68200

Purpose

Provides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Delila coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDsRed (GB0100)

Plasmid

#68202

Purpose

Provides the CDS of DsRed as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

DsRed coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRosea (GB0026)

Plasmid

#68194

Purpose

Provides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

A. majus Rosea coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLH121

Plasmid

#22696

Depositor

Lynn Hudson

Publication

Nadon et al J Neurochem. 1994 Sep . 63(3):822-33.

Insert

Human Proteolipid Protein cDNA (PLP1 Human)

Use

Phagemid

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only
pNP2389 [pNP2314 - ISCro4 Donor + ISCro4 Target-RTG-Matched]

Plasmid

#247251

Purpose

Measure ISCro4 activity via luciferase expression

Depositor

Patrick Hsu

Publication

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP2314 - ISCro4_Donor, ISCro4_Target_RTG-Matched

Expression

Mammalian

Available since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pME-zFUCCI (JDW 1464)

Plasmid

#242558

Purpose

Gateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)

Use

Gateway Cloning

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHygromycin (GB0211)

Plasmid

#68204

Purpose

Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hygromycin resistance gene coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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BII-Sh-FUCCI-Luc

Plasmid

#133364

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

Depositor

Blair Madison

Publication

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Clover-Geminin-IRES-mKO2-Cdt1

Tags

HA-tagged Clover-Geminin

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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