19,662 results

1. pX165-HiFi Cas9

   Plasmid

   #140563

   Purpose

   Expresses HiFi Cas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Mutation

   R691A

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO mScarlet

   Plasmid

   #131002

   Purpose

   Double floxed mScarlet under the control of Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV1 and AAV5

   Insert

   mScarlet
   

   Use

   AAV

   Promoter

   Ef1a

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. HC20 pEYFPN1 anti-Homer1 nanobody

   Plasmid

   #135220

   Purpose

   Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Homer1 (Mouse) recombinant llama nanobody HC20 (Homer1 L. glama (llama))
   

   Tags

   6xHis-HA-EYFP

   Expression

   Mammalian

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xERRE/ERE-luciferase

   Plasmid

   #37852

   Depositor

   Rebecca Riggins

   Article

   Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

   Insert

   3 copies of ERRE/ERE upstream of luciferase
   

   Use

   Luciferase

   Promoter

   SV40

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. hFLT1

   Plasmid

   #83435

   Purpose

   human VEGFR1 with HA and FLAG tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

   Insert

   human VEGFR1 (FLT1 Human)
   

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   HA-tag was inserted 30 AA downstream of the FLT1 …

   Promoter

   CMV

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. EFIa-iTGB1

   Plasmid

   #115799

   Purpose

   Integrin B1 overexpression

   Depositor

   Joan Massague

   Article

   Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

   Insert

   iTGB1 (ITGB1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 AIF 1-90 mCherry FRB

   Plasmid

   #67530

   Purpose

   For expression of mCherry fused FRB to the mitochondrial innermembrane

   Depositor

   Stephen Tait

   Article

   Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

   Inserts

   AIF a.a. 1 - 90
   mCherry
   FRB
   

   Tags

   AIF 1 - 90, FRB, and mCherry

   Expression

   Mammalian

   Mutation

   T->L

   Promoter

   CMV

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMC037-HisMS2_PLP_pac

   Plasmid

   #128233

   Purpose

   Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

   Depositor

   Paul Freemont

   Article

   de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

   Inserts

   Maturation Protein
   Coat Protein Dimer
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 15, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAAV-EF1a-Flpo

   Plasmid

   #55637

   Purpose

   Expresses Flpo in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV1, and AAV2

   Insert

   Flpo
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD/HisB-iRFP670

    Plasmid

    #45453

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Expression

    Bacterial

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    araBAD

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-neo-cOVA

    Plasmid

    #25097

    Depositor

    Maria Castro

    Article

    Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

    Insert

    cytoplasmic ovalbumin
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. hPGK_V5-APEX2-GFP

    Plasmid

    #105285

    Purpose

    drives expression of V5-NES-APEX2-GFP from human PGK promotor

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    V5-APEX2-NES
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    human PGK promoter

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TLCV2-DelGFP

    Plasmid

    #133302

    Purpose

    EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti_MCP-LSD1_Hygro

    Plasmid

    #138457

    Purpose

    lenti vector encoding the MS2-LSD1 with a 2A Hygro resistance marker

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    LSD1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-G-GECO1.2

    Plasmid

    #32446

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.2
    

    Expression

    Mammalian

    Mutation

    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-Rab5A S34N

    Plasmid

    #28045

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab5A (RAB5A Human)
    

    Tags

    EGFP on N-term

    Expression

    Mammalian

    Mutation

    S34N

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1-ACTR3

    Plasmid

    #8462

    Depositor

    Matthew Welch

    Article

    Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

    Insert

    ACTR3 (ACTR3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVITRO1-Trastuzumab-IgG4/κ

    Plasmid

    #61887

    Purpose

    Expresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    

    Expression

    Mammalian

    Promoter

    mEF1 Prom and rEF1 Prom

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-Vimentin-N-18

    Plasmid

    #55158

    Purpose

    Localization: Intermediate Filaments, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Vimentin (VIM Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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