We narrowed to 18,622 results for: Met;
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Plasmid#47843PurposeBIOFAB RFP reporter plasmid for measuring promoter 12 + BCD1 efficiency.DepositorInsertpromoter 12 and BCD1
UseSynthetic BiologyExpressionBacterialPromotersee insertAvailable SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-Puro
Plasmid#86708PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-GPT2-3xFLAG
Plasmid#163449Purposelentiviral overexpression vector for human GPT2DepositorInsertGPT2 (GPT2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-RpHLuorin2
Plasmid#171720PurposeLysosomal expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
Tagsratiometric pHluorin2ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX304
Plasmid#25890Purpose3rd generation lentiviral Gateway destination vector. Blasticidin selection, V5 tag.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vectorTagsV5ExpressionMammalianAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_Giantin_C1
Plasmid#85050PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
Plasmid#71236PurposeExpress sgRNA and dCas9-KRAB from lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A Puro
sgRNA
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-VP64
Plasmid#47107PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cellsDepositorInsertdCas9-VP64
UseCRISPRTagsFlag, HA, SV40 NLS, and VP64ExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVUHshp53-tTR-KRAB
Plasmid#11654PurposeTet-regulated (Tet-on) lentiviral vector for shp53 (hUbiquitin promoter) - 3rd generationDepositorInserthUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO (TP53 Human)
UseCre/Lox, Lentiviral, and RNAiExpressionMammalianAvailable SinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
DLX2-hygro
Plasmid#97330PurposeGenerating lentiviruses to express Dlx2 and hygromycin resistanceDepositorAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only