We narrowed to 8,430 results for: APP

1. pcDNA3.1(+)mGAT1YFP28

   Plasmid

   #41673

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   “Monomerizing” YFP A206K mutation; 28 C-terminal-…

   Promoter

   CMV

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3. Flag-CSL WT

   Plasmid

   #167004

   Purpose

   Mammalian expression of CSL WT

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMX-SHP1C/S-ires-mCD8

   Plasmid

   #183719

   Purpose

   Expresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) (substrate-trapping mutant) and mouse CD8.

   Depositor

   Takeshi Tsubata

   Article

   Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

   Insert

   SHP-1
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mutation

   C453S

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmScarlet-i_Giantin_C1

   Plasmid

   #85050

   Purpose

   In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Giantin (GOLGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 3131-3259

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKb-TnpB1

   Plasmid

   #215333

   Purpose

   ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

   Depositor

   Kutubuddin Molla

   Article

   Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

   Insert

   Rice codon optimized ISDra2 TnpB
   

   Use

   Tags

   Not applicable

   Expression

   Plant

   Mutation

   Promoter

   Rice ubiquitin promoter for TnpB and OsU3 promote…

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX4T2 RANK-L

   Plasmid

   #108571

   Purpose

   Encodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tag

   Depositor

   Jean-Claude Scimeca

   Article

   Beranger et al J Bone Miner Res. 2007 Jul;22(7):975-83.

   Insert

   RANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   aa158-aa316

   Promoter

   Ptac (trp/lac)

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

   Plasmid

   #134865

   Purpose

   Expresses EGFP-tagged Golgi apparatus marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Membrin (GOSR2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHM151Env

   Plasmid

   #139017

   Purpose

   Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

   Depositor

   Anjali Joshi

   Article

   Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

   Insert

   HIV-1 Envelope Glycoprotein (env HIV-1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-Universal.Sth.dCas9

   Plasmid

   #171088

   Purpose

   CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
   DHFR-TSc3
   

   Use

   Toxoplasma gondii expression

   Tags

   Expression

   Mutation

   Note: A S245F mutation is present but did not app…

   Promoter

   DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5FRT/TO-Cezanne-Strep-HA

    Plasmid

    #113492

    Purpose

    Expression of human Cezanne with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    Cezanne (OTUD7B Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMIR-21 Reporter

    Plasmid

    #71870

    Purpose

    Mammalian expression vector for the analysis of miR-21 activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-21
    

    Use

    Tags

    firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

    Plasmid

    #99229

    Purpose

    Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos2
    

    Use

    Tags

    Lifeact

    Expression

    Mammalian

    Mutation

    A69T for primed conversion applications

    Promoter

    CMV

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHM106Env

    Plasmid

    #139024

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein (env HIV-1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. NES-PAmCherry-cPHx3

    Plasmid

    #116861

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    PAmCherry

    Expression

    Mammalian

    Mutation

    amino acids 169-329 (tandem trimer)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. BII-gR-BtW-eSpCas9

    Plasmid

    #133358

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Use

    Tags

    Flag-tagged eSpCas9

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)mGAT1YFP8

    Plasmid

    #41669

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; 8 C-terminal-m…

    Promoter

    CMV

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSET-pr-mKikGR1 (mKikGR1-V69T)

    Plasmid

    #99227

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mKikGR1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    monomeric variant with mutation V69T (Eos notatio…

    Promoter

    T7

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmScarlet-H_Giantin_C1

    Plasmid

    #85049

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Giantin (GOLGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 3131-3259

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. 139H2_LC

    Plasmid

    #206202

    Purpose

    For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.

    Depositor

    Joost Snijder

    Article

    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

    Insert

    anti-MUC1 antibody 139H2 light chain (Igkc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. iBE3 CLYBL

    Plasmid

    #174569

    Purpose

    doxycycline inducible BE3 expression for human CLYBL locus knock-in

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet ON

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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