We narrowed to 22,939 results for: REP
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Plasmid#203886PurposeViral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.DepositorInsertCHLH fragment
UseRNAiExpressionPlantAvailable SinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_A467
Plasmid#216234PurposeS. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged alanine 467 to valinePromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_T522
Plasmid#216235PurposeS. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged threonine 522 to isoleucinePromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
2x3APWWP-IFP2.0C
Plasmid#215752PurposeExpresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensorsDepositorInsert2x3APWWP-IFP2.0C
ExpressionMammalianPromoterCMVAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA
Plasmid#174897Purposeochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassetteDepositorInsertsf GFP
ExpressionMammalianMutation150TAA in sfGFPPromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA
Plasmid#174898Purposeopal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassetteDepositorInsertsfGFP
ExpressionMammalianMutation150TGA in sfGFPPromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRV∆L-5tTA
Plasmid#182966PurposeThe 3rd generation of rabies vector expressing a tetracycline-controlled transactivator (tTA)DepositorInserttTA
UseRabiesAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG.Myc-SARS2_3CL(C145A)
Plasmid#200121PurposeExpresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cellsDepositorInsertSARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
TagsMycExpressionMammalianMutationC145APromoterCAGAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TERTp124_Gluc_CCR5
Plasmid#192271PurposeDonor template for insertion of hTERT promoter (-124 C to T) driven Gaussia luciferase reporterDepositorInsertPromoter Reporter Insert within donor template
UseCRISPRMutation-124 C to TPromoterTERT promoter driving expression of gaussiaAvailable SinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TERTp146_Gluc_CCR5
Plasmid#192272PurposeDonor template for insertion of hTERT promoter (-146 C to T) driven Gaussia luciferase reporterDepositorInsertPromoter Reporter Insert within donor template
UseCRISPRMutation-146 C to TPromoterTERT promoter driving expression of gaussiaAvailable SinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBA-Con/Fon-EYFP-WPRE
Plasmid#186752PurposeAAV-mediated Cre and Flp-dependent expression of EYFP under CBA promoterDepositorInsertEYFP
UseAAVPromoterCBAAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry-SynaptoZip
Plasmid#177318PurposeAAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoterDepositorInsertmCherry-SynaptoZip (Vamp2 Rat)
UseAAVTagsMyc and mCherryExpressionMammalianPromoterhSynAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
MO70-C-FLAG-hNKD2
Plasmid#174327Purposeencodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistanceDepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseRetroviralTagsFLAG TagExpressionMammalianPromoterCMVAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS5-TVA-eGFP-N2cG
Plasmid#175440PurposeCre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoerDepositorInsertTVA-P2A-eGFP-P2A-N2cG
UseAAV and Cre/LoxAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pColE1-AlphaOmega
Plasmid#165867PurposeTerminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG418R-MCS-LexA-VP16-MiniWhite
Plasmid#165894PurposeG418 resistant LexA driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.DepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Alpha1
Plasmid#165858PurposeAlpha1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Omega1
Plasmid#165860PurposeOmega1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Alpha2
Plasmid#165863PurposeAlpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcry:mCherry,-600unc:cct3GFP
Plasmid#172528PurposealphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.DepositorInsertcct3-GFP
ExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only