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pDONR223 PAK5

Plasmid

#60532

Purpose

Donor vector for human Pak5

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway Cloning

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
PCXGW

Plasmid

#48257

Purpose

Split-GFP assay in plants

Depositor

Wolf Frommer

Article

Kim et al Plant Cell. 2009 Apr;21(4):1305-23. doi:

Type

Empty backbone

Use

Binary gateway vector

Tags

6xHIS and CFP aa155-238

Promoter

35S

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
mTagBFP2-MCHR1-N-10

Plasmid

#55313

Purpose

Localization: Cilium, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MCHR1

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pX28

Plasmid

#46850

Purpose

TOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiency

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB, RFP and PGK-puro

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGS-TAL-NC2

Plasmid

#43856

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Tags

Flag and FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2+ tecktin-GFP

Plasmid

#26932

Depositor

Christopher Kintner

Article

Park et al Nat Genet. 2008 Jul . 40(7):871-9.

Insert

Tektin 2 (tekt2 Frog)

Tags

GFP

Expression

Mammalian

Mutation

I429A

Available Since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only
pTE-0X38-GCT1

Plasmid

#27053

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-gfp-ssrAec

Expression

Bacterial

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pKS101

Plasmid

#24631

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

FAA2 (FAA2 Budding Yeast)

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pDUET-ctCPR-A13AMO

Plasmid

#20114

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
ZP45 Prickle-1

Plasmid

#16897

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pKC092_pLenti_TetON_QtEnc_IRES_RRM34_QtTP

Plasmid

#242120

Purpose

Tet- or Dox-inducible expression of QtEnc and RRM34 fused to QtTP

Depositor

Fei Chen

Article

Chao et al Science. 2026 Jan 15:eadz9353. doi: 10.1

Insert

QtEnc and PABPC1 RRM34_QtTP (PABPC1 Human)

Use

Lentiviral

Mutation

PABPC1 aa 191-470

Promoter

TRE3G

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP

Plasmid

#233353

Purpose

CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CD4-sTb(C)-M13-GFP

Plasmid

#219780

Purpose

sTb(C) half CaST for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTb(C)-M13-GFP

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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B2396_BTB-EGFP

Plasmid

#215099

Purpose

Expresses BCL6 BTB(1-129)-EGFP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BTB-EGFP (BCL6 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAG1171 FIREmate-ECFP-Giantin

Plasmid

#205166

Purpose

Expresses FIREmate-ECFP-Giantin in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Giantin

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMUT2-pTcpP-mCherry

Plasmid

#192861

Purpose

pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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Clathrin-mhYFP

Plasmid

#186527

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to clathrin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

Clathrin

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2

Plasmid

#183352

Purpose

Flp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799)

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

TVA, EGFP and tTA

Use

AAV

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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dCas9-NLS-gs10-cERb447

Plasmid

#188257

Purpose

Plasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

cERb447 (ESR1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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dCas9-NLS-gs10-cTRb413

Plasmid

#188261

Purpose

Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

cTRb413 (TXNRD2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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