19,662 results

1. pRPF215

   Plasmid

   #106377

   Purpose

   C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

   Depositor

   Robert Fagan, Neil Fairweather

   Article

   Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

   Insert

   Ptet-himar1-Ter(slpA)-transposon
   

   Use

   E. coli - c. difficile shuttle vector

   Mutation

   Codon optimised for C. difficile

   Promoter

   Ptet

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti CMV/TO GFP-Zeo DEST (719-1)

   Plasmid

   #17431

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDuet TDP43 WT

   Plasmid

   #27462

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Coexpression

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV5B-Flag-Smurf2 wt

   Plasmid

   #11746

   Depositor

   Jeff Wrana

   Article

   Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

   Insert

   SMURF2 (SMURF2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pMOD_A1110

   Plasmid

   #91031

   Purpose

   Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TaCas9
   

   Use

   CRISPR

   Promoter

   ZmUbi

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTag-BFP-C-h-Rab7L1-c-Myc

   Plasmid

   #79803

   Purpose

   Express TagBFP labeled human Rab7L1.

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   Rab7L1 (RAB29 Human)
   

   Tags

   TagBFP and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. stathmin-GFP

   Plasmid

   #86782

   Purpose

   Mammalian expression of stathmin-GFP

   Depositor

   Lynne Cassimeris

   Article

   Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

   Insert

   stathmin (STMN1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSP64T (DM#111)

   Plasmid

   #15030

   Depositor

   Douglas Melton

   Article

   Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

   Insert

   beta-globin 5' and 3' UTR
   

   Expression

   Bacterial

   Available Since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB-TAC-ERN

   Plasmid

   #80475

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Expression

   Mammalian

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BRCA1 A10.2 gRNA

    Plasmid

    #90549

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA1 (Guide Designation A10.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPR-Cas9-ScaligD

    Plasmid

    #125688

    Purpose

    Gene knockout/in for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    ermE*/tipA

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-SypHluorin 4x (S4x)

    Plasmid

    #37005

    Depositor

    Stephen Heinemann, Yongling Zhu

    Article

    Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

    Insert

    SypHluorin 4x (Syp Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Four copies of pHluorin inserted at site 184-185

    Promoter

    CMV

    Available Since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. eGFP STAT1 S727A

    Plasmid

    #12304

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

    Insert

    STAT1 S727A (STAT1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S727A

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRB1017

    Plasmid

    #59936

    Purpose

    empty vector for gRNA cloning

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-dCas9-FLp300

    Plasmid

    #61356

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET41b+_Npl4

    Plasmid

    #117108

    Purpose

    Production of recombinant Npl4 in E.coli

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    Npl4 (NPLOC4 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psiCHECK2-let-7 8x

    Plasmid

    #20931

    Depositor

    Yukihide Tomari

    Article

    Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

    Insert

    8x let-7 target sites
    

    Use

    Luciferase

    Expression

    Insect and Mammalian

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTriEx4-Cdc42-2G

    Plasmid

    #68814

    Purpose

    FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

    Depositor

    Olivier Pertz

    Article

    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

    Insert

    Cdc42 activation reporter
    

    Tags

    His and Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-ERK2

    Plasmid

    #37145

    Depositor

    Rony Seger

    Article

    Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

    Insert

    ERK2 (MAPK1 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-6 deleted as a result of cloning strategy

    Promoter

    CMV

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

20. dCas-hHDAC3-R265P

    Plasmid

    #103786

    Purpose

    Expresses dCas9 fused to human HDAC3 with R265P mutation

    Depositor

    Zhaolan Zhou

    Article

    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

    Insert

    dCas9-HDAC3-R265P (HDAC3 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Changed arginine 265 to proline

    Promoter

    EF1A

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge