We narrowed to 10,108 results for: Coli
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Plasmid#61583PurposeExpresses human VCPIP (OTU domain) in E. coli.DepositorInsertVCPIP (VCPIP1 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-24 and aa 562-1222.PromoterT7Available SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorT(sp)-mNeonGreen
Plasmid#205021PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorT-mNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-ORF3b (S23Q)
Plasmid#165088Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
ExpressionBacterialMutationStop codon to glutamine at 23rd amino acidAvailable SinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPINK-OTUD1 (OTU, aa 287-437)
Plasmid#61407PurposeExpresses human OTUD1 (OTU domain) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-286 and aa 438-481.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Sos1 (PRM) - Cys (XSB364)
Plasmid#78506PurposeConstruct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labelingDepositorInsertSos1 -Cys (PRM) (SOS1 Human)
TagsGST followed by a prescission protease claevage s…ExpressionBacterialAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUB2 (full-length, aa 1-234)
Plasmid#61421PurposeExpresses human OTUB2 (full-length) in E. coli.DepositorInsertOTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)
TagsHis6-GST-3CExpressionBacterialPromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-ORF8
Plasmid#165089Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
ExpressionBacterialAvailable SinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRD395
Plasmid#163102PurposeExpression of mCherry_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmCherry_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains1
Plasmid#78461PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
TagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2149-2170; Q2513R (please see…PromoterT7Available SinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP3
Plasmid#129501PurposeExpresses AausFP3 constitutively in E. coli (most strains)DepositorInsertAausFP3
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains2
Plasmid#78460PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
TagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2264-2315; Q2513R (see deposi…Available SinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS3
Plasmid#140626PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS3
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP1-C
Plasmid#128311PurposeExpress hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tagDepositorInsertHuman nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)
TagsHisExpressionBacterialPromoterT7Available SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP4
Plasmid#129502PurposeExpresses AausFP4 constitutively in E. coli (most strains)DepositorInsertAausFP4
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-LplA(AAG)
Plasmid#61820PurposeContains GFP tag for direct live-cell fluorescence imaging of the resofurin ligaseDepositorInsertE. coli lipoic acid ligase
TagsGFPExpressionMammalianMutationE20A, F147A, H149GPromoterCMVAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD6A (OTU, aa 128-288)
Plasmid#61417PurposeExpresses human OTUD6A (OTU domain) in E. coli.DepositorInsertOTUD6A (OTUD6A Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-127.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD6B (OTU, aa 133-293)
Plasmid#61419PurposeExpresses human OTUD6B (OTU domain) in E. coli.DepositorInsertOTUD6B (OTUD6B Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-132.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM
Plasmid#107163PurposeExpresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzymeDepositorInsertF37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
TagsGSTExpressionBacterialPromotertacAvailable SinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-Y2404F
Plasmid#182846Purposemutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG inductionDepositorInsertTRR 2011-2431 (trr Fly)
ExpressionBacterialMutationmutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…Available SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAR69_STRI_m(KS_MAT)_H8_pET22b
Plasmid#122849PurposeExpresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInsertm(KS_MAT) (Fasn Mouse)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only