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mRFP1-N1

Plasmid

#54635

Purpose

Localization: N1 Cloning Vector, Excitation: 584, Emission: 607

Depositor

Roger Tsien, Robert Campbell, Michael Davidson

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Use

Tags

mRFP1

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pEnt L1L3 rtTA-3

Plasmid

#27106

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

reverse tetracycline transactivator

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-LgBiT-SpyCatcher

Plasmid

#223634

Purpose

Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Use

Tags

His6, LgBiT

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK4 wt

Plasmid

#80879

Purpose

mammalian expression of ALK4 WT

Depositor

Peter ten Dijke, Aristidis Moustakas

Article

ten Dijke et al Science. 1994 Apr 1;264(5155):101-4.

Insert

ALK4 (ACVR1B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Tp-gfpmut3

Plasmid

#65033

Purpose

TpR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

gfpmut3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcs2-HRI-GFP-IRES-mCherry

Plasmid

#226099

Purpose

HRI stability reporter construct for transient expression in mammalian cells

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

HRI (EIF2AK1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pSNAPf-N1

Plasmid

#58187

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLenti-CMV-cGASΔN-HA

Plasmid

#130912

Purpose

Expresses human cGASΔN (aa160-522)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGASΔN

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-SpRY-His

Plasmid

#179320

Purpose

Plasmid for bacterial expression and purification of SpRY-Cas9

Depositor

Julián Cerón, Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Human codon-optimized Cas9 variant SpRY

Use

CRISPR

Tags

6xHis and SV40-NLS

Expression

Bacterial

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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iC10 PB-zim3-mycNLS-mApple-hygro

Plasmid

#204719

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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