We narrowed to 11,495 results for: ANG
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BE3-YE1
Plasmid#132943PurposeGene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.DepositorInsertBE3-(W90Y-R126E)
UseCRISPR; Base editorExpressionMammalianMutationBE3-(W90Y-R126E)Available SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - DANCER-1
Plasmid#136385PurposeExpresses DANCER-1 dynamic proteinDepositorInsertDANCER-1
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L-V54IPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - DANCER-2
Plasmid#136386PurposeExpresses DANCER-2 dynamic proteinDepositorInsertDANCER-2
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L5A-L7I-A34F-V39LPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - NERD-S
Plasmid#136388PurposeExpresses NERD-S static proteinDepositorInsertNERD-S
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-A34F-V39I-V54IPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIS-rPALcc
Plasmid#145804Purposeexpresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cellsDepositorInsertrat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
ExpressionMammalianMutationchanged serine 767 to alanine to prevent glycosyl…PromoterCMVAvailable SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIs8-4b-SaDAH::G226D
Plasmid#140130PurposeExpresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase G226D variant from Sinomenium acutum
Tags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationchanged Gly226 to Asp226PromoterT7Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE Y165A
Plasmid#139428PurposeGST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged tyrosine 165 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE P199A
Plasmid#139427PurposeGST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged proline 199 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE I137A
Plasmid#139319PurposeGST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged isoleucine 137 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2 (D30N)
Plasmid#134289PurposeExpresses GK5 (isoform 2)-D30N mutant in bacteriaDepositorInsertGk5 (Gk5 Mouse)
TagsMBPExpressionBacterialMutationchanged Aspartic Acid 30 to AsparaginePromoterT7Available SinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-Snrnp40
Plasmid#134255PurposeLentivector for Snrnp40 CRISPR knockoutDepositorInsertSnrp40 (Snrnp40 Mouse)
UseCRISPR and LentiviralMutationSnrnp40 gRNA “GATAACTATGCGACGTTGAA”PromoterU6Available SinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ad-MKP-3 C293S
Plasmid#131525PurposeExpresses MKP-3 with C293S mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoMutationchanged Cysteine 293 to SerinePromoterCMVAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 ZFAND3-guide2
Plasmid#125514PurposeCRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralExpressionMammalianPromoterEF1a core and U6Available SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 ZFAND3-guide1
Plasmid#125513PurposeCRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralExpressionMammalianPromoterEF1a core and U6Available SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 rs58692659-guide4
Plasmid#125512PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralExpressionMammalianPromoterEF1a core and U6Available SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 rs58692659-guide3
Plasmid#125511PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralExpressionMammalianPromoterEF1a core and U6Available SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 rs58692659-guide2
Plasmid#125510PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralExpressionMammalianPromoterEF1a core and U6Available SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 rs58692659-guide1
Plasmid#125509PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralExpressionMammalianPromoterEF1a core and U6Available SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 rs7732130-guide3
Plasmid#125505PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralExpressionMammalianPromoterEF1a core and U6Available SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only
