We narrowed to 29,897 results for: des.2

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Puro-TTL-wt-TTF-1

Plasmid

#67657

Purpose

To create a dox-on inducible expression of human wt-TTF-1 cDNA in cells

Depositor

David Mu

Article

Rice et al J Biol Chem. 2013 Jun 7;288(23):16348-60

Insert

Thyroid Transcription Factor 1 (NKX2-1 Human)

Use

Retroviral

Tags

N/A

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBacHTA-NEDD4L_FL

Plasmid

#187722

Purpose

Express His-tagged NEDD4L FL in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL (NEDD4L Human)

Tags

His-TEV

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_FL

Plasmid

#187705

Purpose

Express his-tagged NEDD4L FL in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-83-R56ER63ER81E-GB1_AB

Plasmid

#148378

Purpose

Bacterial Expression of DmThor_50-83-R56ER63ER81E

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_50-83-R56ER63ER81E (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-83-R63E-GB1_AA

Plasmid

#148273

Purpose

Bacterial Expression of DmThor_50-83-R63E

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_50-83-R63E (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-83-YMVLtoAADD-GB1_AA

Plasmid

#148274

Purpose

Bacterial Expression of DmThor_50-83-YMVLtoAADD

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_50-83-YMVLtoAADD (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_32-83-S65DT70D-GB1_AA

Plasmid

#148268

Purpose

Bacterial Expression of DmThor_32-83-S65DT70D

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_32-83-S65DT70D (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_32-83-YMSTtoAADD-GB1_AA

Plasmid

#148269

Purpose

Bacterial Expression of DmThor_32-83-YMSTtoAADD

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_32-83-YMSTtoAADD (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-83-R56AR63A-GB1_AA

Plasmid

#148270

Purpose

Bacterial Expression of DmThor_50-83-R56ER63A

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_50-83-R56ER63A (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-83-R56E-GB1_AA

Plasmid

#148271

Purpose

Bacterial Expression of DmThor_50-83-R56E

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_50-83-R56E (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-83-R56ER63E-GB1_AA

Plasmid

#148272

Purpose

Bacterial Expression of DmThor_50-83-R56ER63E

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_50-83-R56ER63E (Thor Fly)

Expression

Bacterial

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pACgp67B-Her2

Plasmid

#10794

Depositor

Judy Lieberman

Article

Song et al Nat Biotechnol. 2005 Jun . 23(6):709-17.

Insert

ErbB2 ScFv (ERBB2 Human)

Tags

6His

Expression

Insect

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-LMW-FGF2

Plasmid

#157663

Purpose

Expresses FGF2 in mammalian cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Tags

HA tag

Expression

Mammalian

Mutation

deleted amino acid 1-134

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_HECT

Plasmid

#187719

Purpose

Express GST-tagged NEDD4L HECT domain in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 594-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-593

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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TPC2(N257A)-mCherry

Plasmid

#135186

Purpose

Pore-dead mutant of TPC2, tagged on C terminus with mCherry.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Mutation of asparagine257 to alanine

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTET2 (CD)

Plasmid

#89736

Purpose

Expresses the CD-domain truncation to mouse TET2 in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

TET2 (Tet2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

CD (cysteine-rich, dioxygenase domain) truncation…

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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TPC2(E643A)-mCherry

Plasmid

#135187

Purpose

Mutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Mutation of glutamate 643 to alanine

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pHis-LMW_FGF2

Plasmid

#157657

Purpose

Expresses FGF2 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Tags

His tag

Expression

Bacterial

Mutation

changed Cystein 211,229 to serine, deleted amino …

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L_C942S_pCDNA3.1(+)

Plasmid

#187729

Purpose

Express the catalytically inactive NEDD4L FL in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

Cys874 mutated to a serine

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Tcf4

Plasmid

#139806

Purpose

doxycycline-inducible expression of mouse Tcf4 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Tcf4 (Tcf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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