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pMuLE ENTR CMV iRFP R4-R3

Plasmid

#62158

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

iRFP

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 eGFP L5-L2

Plasmid

#62144

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and EGFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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Abr DH domain NE/AA pGEX

Plasmid

#38182

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

ABR DH domain N282AE283A (ABR Human)

Tags

GST

Expression

Bacterial

Mutation

ABR DH domain with N282A and E283A mutations

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
Abr DH domain pGEX

Plasmid

#38181

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

ABR DH domain (ABR Human)

Tags

GST

Expression

Bacterial

Mutation

ABR DH domain

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
myr-Abr

Plasmid

#38174

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

ABR transcript variant 2 (ABR Human)

Expression

Mammalian

Mutation

addition of a Src myristoylation sequence (see co…

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
Abr GAP domain /pGEX 3X

Plasmid

#36407

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

Abr GAP domain (ABR Human)

Tags

GST

Expression

Bacterial

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
BRCA1-(936-1113)

Plasmid

#35075

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Mutation

Contains aa 936-1113

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
BRCA1-(1113-1316)

Plasmid

#35077

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Mutation

Contains aa 1113-1316

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
BRCA1-(894-1057)

Plasmid

#35074

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Mutation

Contains aa 894-1057

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
GFP-GW1D1aMut(W>A)

Plasmid

#21522

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa254-503, mutate every tryptophan (total…

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
CSAC-Crys

Plasmid

#164967

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2

Use

AAV

Tags

mCherry

Promoter

hU6, hSyn

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0396-luc2

Plasmid

#227115

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0396

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0396 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_CRE-CMVmin-BC0481-luc2

Plasmid

#227132

Purpose

Barcoded assay, cAMP/Ca2+ sensor; barcode BC0481

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

6x clustered CRE element linked to CMV minimal promoter driving barcode 0481 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME EFS-rtTA/rtTA3 (JDW 1230)

Plasmid

#224525

Purpose

A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EFS-rtTA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mCardinal (JDW 1418)

Plasmid

#242553

Purpose

Gateway middle entry clone containing H2B-mCardinal; Nuclear far red fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCardinal

Use

Gateway subcloning

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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p5E TRE-3GV (JDW 1163)

Plasmid

#224476

Purpose

Gateway compatible 5' entry clone containing a 3rd generation Tet-On promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TRE3GV promoter

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Il12b 400bp pro-Luc

Plasmid

#20020

Depositor

Stephen Smale

Article

Plevy et al Mol Cell Biol. 1997 Aug . 17(8):4572-88.

Insert

Il12b promoter (Il12b Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV_ENVLPE+

Plasmid

#232427

Purpose

Expression plasmid to produce ENVLPE+ particles

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

ENVLPE+

Expression

Mammalian

Promoter

2xCMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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TA1-DuET

Plasmid

#213382

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

TA1 (TAAR1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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