We narrowed to 22,800 results for: promoter

1. pLKO.1 puro shYAP1-1

   Plasmid

   #193692

   Purpose

   Constitutive lentiviral expression of YAP1 shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Human FLAG-UAF1 S170Y

   Plasmid

   #217619

   Purpose

   Mammalian expression of FLAG tagged mutant (UAF1 S170Y) UAF1

   Depositor

   Ethan Lee

   Article

   Ng et al Nat Commun. 2023 Oct 5;14(1):6173. doi:

   Insert

   UAF1 (WDR48 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S170Y

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. R26-dCas9-VPR-mNG

   Plasmid

   #172441

   Purpose

   Rosa26 targeting vector, expresses dCas9-VPR and mNeonGreen

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Inserts

   dCas9-VPR
   mNeonGreen
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29(+exo) (LM2990)

   Plasmid

   #208958

   Purpose

   A variant CE1 construct with ePhi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-ePhi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); ePhi29(M8R/V51A/M97T/G197D/E221K/…

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TRE-DIO-ChR2-EYFP

   Plasmid

   #183765

   Purpose

   This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene -teto promoter-flex/dio-ChR2-EYFP
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   ChR2-EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   tetO promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

   Plasmid

   #210710

   Purpose

   This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   M11

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-HNF1B-WT-Flag-UTR

   Plasmid

   #183239

   Purpose

   Expression of FLAG tagged HNF1B

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   HNF1 homeobox B (HNF1B Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRE3GV-SpdCas9-Dnmt3A/3L-WT

   Plasmid

   #222502

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   TRE3GV and hPGK

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKrox24(MapERK)d1EGFP (KroxDs)

   Plasmid

   #214912

   Purpose

   for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation

   Depositor

   Pavel Krejčí

   Article

   Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

   Insert

   d1EGFP under the MapERK promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MapkERK promoter

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRECLV103_PGBD5_B

    Plasmid

    #168053

    Purpose

    Lentiviral expression vector for fusion of GFP with human PGBD5, NCBI: NM_001258311.1

    Depositor

    Alex Kentsis

    Article

    Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

    Insert

    PGBD5 (PGBD5 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-GFAP-eGFP-shLacZ

    Plasmid

    #223222

    Purpose

    mir30 based shRNA strategy, control shRNA

    Depositor

    Li-Huei Tsai

    Article

    Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

    Insert

    shLacZ
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

    Plasmid

    #168305

    Purpose

    mediated knock-in of sgRNA precursor

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    SaKKH-EGFP-U6-sgRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha, CBh

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-U6-sgMettl3

    Plasmid

    #196263

    Purpose

    Expresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoter

    Depositor

    Laura Attardi

    Article

    Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

    Insert

    Mettl3 sgRNA (Mettl3 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX317-MBNL2

    Plasmid

    #115444

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    MBNL2 (MBNL2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPL001-BBTK

    Plasmid

    #184751

    Purpose

    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    pEF1a-eGFP-T2A-BSD-bGHpA
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-HsBIN1_tv8

    Plasmid

    #176012

    Purpose

    Mammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBH

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-noSV40-humanCOL2A1enhancer

    Plasmid

    #173961

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Enhancer region from COL2A1 gene
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    SV40 promoter

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSYC-437

    Plasmid

    #163829

    Purpose

    Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

    Depositor

    Seok-Yong Choi

    Article

    Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

    Inserts

    Foxj1a Promoter
    GCaMP6s
    

    Use

    Multisite gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCRISPRv2-NONO 40

    Plasmid

    #127653

    Purpose

    Knock-out of human NONO

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

    Insert

    NONO sgRNA (NONO Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3 2 kb prom. and exon 1 fragment CD274

    Plasmid

    #107007

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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