We narrowed to 18,937 results for: Tec

1. SG3ΔENV CA Q67H/N74D

   Plasmid

   #217442

   Purpose

   Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D).

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   gag/pol
   

   Use

   Lentiviral

   Mutation

   CA Q67H,N74D

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+ 3xFLAG-FEM1B C186S

   Plasmid

   #230995

   Purpose

   Transient overexpression of mouse FEM1B in mammalian cells. Mutation abolishes substrate binding.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Insert

   FEM1B (Fem1b Mouse)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   mutated Cys186 to Ser

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+ 3xFLAG-FEM1B L597A

   Plasmid

   #230994

   Purpose

   Transient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Insert

   FEM1B (Fem1b Mouse)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   mutated Leu597 to Ala

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. CROP-seq-copGFP-v2-no-scaffold

   Plasmid

   #228520

   Purpose

   CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

   Depositor

   Jellert Gaublomme

   Article

   Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-RhoA*

   Plasmid

   #198716

   Purpose

   Expresses gRNA resistant RhoA under the CAG promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoA (Rhoa Mouse)
   

   Expression

   Mammalian

   Mutation

   It has mutations that is resistant to the RhoA gR…

   Promoter

   CAG

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9-NRCH-CGBE1

   Plasmid

   #198557

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-CGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-NG-ABE8.17-m+V106W

   Plasmid

   #198534

   Purpose

   Expresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-ABE8.17-m+V106W
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST17-DXO

   Plasmid

   #177993

   Purpose

   For purification of N-terminally His-tagged DXO from bacteria

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   DXO (DXO Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE3G-marmoset cGASdeltaN-HA

   Plasmid

   #186863

   Purpose

   Doxycycline-dependent expression of marmoset cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Marmoset cGAS truncation mutant (160-547 a.a.) with C-terminal HA tag
   

   Use

   Retroviral

   Tags

   HA

   Mutation

   N-terminal truncation

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE3G-WHG cGASdeltaN-HA

    Plasmid

    #186868

    Purpose

    Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    White-handed gibbon cGAS truncation mutant (193-557 a.a.) with C-terminal HA tag
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)

    Plasmid

    #225441

    Purpose

    Module for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.

    Depositor

    Diego Orzaez

    Article

    Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

    Insert

    hygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAPM-miR30-XPO1-ts1

    Plasmid

    #174286

    Purpose

    XPO1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    XPO1 shRNA (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAPM-miR30-XPO1-ts2

    Plasmid

    #174287

    Purpose

    XPO1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    XPO1 shRNA (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAPM-miR30-XPO1-ts3

    Plasmid

    #174288

    Purpose

    XPO1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    XPO1 shRNA (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAPM-miR30-MATR3-ts1

    Plasmid

    #174271

    Purpose

    MATR3 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MATR3 shRNA (MATR3 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAPM-miR30-MATR3-ts2

    Plasmid

    #174272

    Purpose

    MATR3 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MATR3 shRNA (MATR3 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAPM-miR30-MATR3-ts3

    Plasmid

    #174273

    Purpose

    MATR3 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MATR3 shRNA (MATR3 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAPM-miR30-HNRNPR-ts3

    Plasmid

    #174255

    Purpose

    HNRNPR knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    HNRNPR shRNA (HNRNPR Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAPM-miR30-ILF2-ts1

    Plasmid

    #174262

    Purpose

    ILF2 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    ILF2 shRNA (ILF2 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAPM-miR30-ILF2-ts2

    Plasmid

    #174263

    Purpose

    ILF2 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    ILF2 shRNA (ILF2 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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