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pRSETa-mKikGR-V69T

Plasmid

#98574

Purpose

photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mKikGR-V69T

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETb-pcDronpa2-A69T

Plasmid

#98578

Purpose

photoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

pcDronpa2-A69T

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dgcr8_1

Plasmid

#73525

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pCfB3043(gRNA XI-1)

Plasmid

#73285

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pP2AeGFP-Mars1Cy

Plasmid

#75223

Purpose

Mammalian intracellular expression of fluorogen activating protein Mars1Cy and eGFP

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

EGFP-p2a-Mars1Cy

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCMB-TPr

Plasmid

#61190

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-PMr

Plasmid

#61181

Purpose

Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPIP2a

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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mRuby2-Tubulin-6

Plasmid

#55914

Purpose

Localization: Microtubules, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Tubulin (TUBA1B Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
tdTomato-Dectin1A-C-10

Plasmid

#58088

Purpose

Localization: Membrane, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

Dectin1A (CLEC7A Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-MannII-N-10

Plasmid

#55429

Purpose

Localization: Mannosidase/Golgi, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

MannII

Tags

mCerulean3

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-EB1-C-18

Plasmid

#55415

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB1 (MAPRE1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-SSTR3-N-17

Plasmid

#55446

Purpose

Localization: Cilium, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

SSTR3

Tags

mCerulean3

Expression

Mammalian

Mutation

A270V in SSTR3

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean-Fibrillarin-7

Plasmid

#55368

Purpose

Localization: Nucleoli, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Insert

Fibrillarin (FBL Human)

Tags

mCerulean

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mAzurite-Cx32-7

Plasmid

#55230

Purpose

Localization: Gap Junctions, Excitation: 384, Emission: 450

Depositor

Michael Davidson

Insert

Cx32 (GJB1 Human)

Tags

mAzurite

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
CT-Nano-lantern(ATP1)/pRSETB

Plasmid

#51990

Purpose

bacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Tags

6xHis and chloroplast transit peptid

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pHIS3p:Venus-Tub1+3'UTR::LEU2

Plasmid

#50644

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

Venus-Tub1

Use

Yeast expression

Tags

Venus

Promoter

HIS3p

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pHIS3p:mEos2-Tub1+3'UTR::TRP1

Plasmid

#50652

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mEos2-Tub1

Use

Yeast expression

Tags

mEos2

Promoter

HIS3p

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pHIS3p:mTurquoise2-Tub1+3'UTR::TRP1

Plasmid

#50647

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mTurquoise2-Tub1

Use

Yeast expression

Tags

mTurquoise2

Promoter

HIS3p

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pHIS3p:mEos2-Tub1+3'UTR::HPH

Plasmid

#50634

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mEos2-Tub1

Use

Yeast expression

Tags

mEos2

Promoter

HIS3p

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pBS chick PEA3 [TJ#51]

Plasmid

#16279

Depositor

Thomas Jessell

Article

Lin et al Cell. 1998 Oct 30. 95(3):393-407.

Insert

PEA3 (ETV4 Chicken)

Expression

Bacterial

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only