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pAAV-hsyn-NE2h

Plasmid

#208687

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Has Service

AAV5

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2h

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING

Plasmid

#102586

Purpose

Lentivector to express STING and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

Plasmid

#90015

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-GFP

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer

Plasmid

#90013

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-Dicer

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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SacI ML GFP Strand 11 Long

Plasmid

#164120

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Long

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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SacI ML GFP Strand 11 Short

Plasmid

#164121

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Short

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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PJ-INPP5E

Plasmid

#38001

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

FKBP1A (FKBP1A Human)
SAC1 (SAC1 Budding Yeast)
INPP5E (INPP5E Human)

Tags

mRFP

Expression

Mammalian

Mutation

Cys 779 mutated to Ser

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187951

Purpose

FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBS31-cCARLIN

Plasmid

#160928

Purpose

Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)

Depositor

Fernando Camargo

Article

Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.

Insert

CARLIN gRNAs and CAG-GFP-CARLIN array

Use

Mouse Targeting

Expression

Mammalian

Promoter

U6 promoter for gRNAs; CAG promoter for GFP

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3_erGAP3

Plasmid

#78118

Purpose

Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

Depositor

Teresa Alonso, Javier García-Sancho

Article

Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

Insert

erGAP3

Tags

KDEL and calreticulin signal peptide

Expression

Mammalian

Mutation

S175G, D180Y, I167V and L15Q in GFP moiety and D…

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EPG

Plasmid

#117978

Purpose

Express EPG in mamalian cells

Depositor

Assaf Gilad

Article

Krishnan et al Sci Rep. 2018 Jun 8;8(1):8764. doi: 10.1

Insert

Electromagnetic perceptive gene

Tags

HIS and v5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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CS2+MYC-Etv5:T139D

Plasmid

#49097

Purpose

expression of Etv5 T139D mutant

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv5 T139D (etv5a Zebrafish)

Use

Xenopus/avian/zebrafish

Tags

MYC

Expression

Mammalian

Mutation

T139D

Promoter

CMV

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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PJ-Sac

Plasmid

#38000

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

FKBP1A (FKBP1A Human)
SAC1 (SAC1 Budding Yeast)
INPP5E (INPP5E Human)

Tags

mRFP

Expression

Mammalian

Mutation

Asp 1263 mutated to Ala

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-HA-BACE1

Plasmid

#165032

Purpose

HA-BACE1

Depositor

Juan Bonifacino

Article

Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

Insert

BACE1 (BACE1 Human)

Tags

HA (embedded after propeptide cleavage site)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_TNIK

Plasmid

#45276

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TNIK (TNIK Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 LRP5

Plasmid

#115907

Purpose

Expresses human LRP5 in mammalian cells.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

LRP5 (LRP5 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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VE-cad-eGFP Donor plasmid

Plasmid

#92309

Purpose

Knock eGFP into human VE-cad locus

Depositor

Sean Palecek

Article

Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

Insert

VEcad Homologous Recombination Arms (CDH5 Human)

Use

CRISPR

Expression

Mammalian

Mutation

about 30 a.a. truncated at end of protein

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A-nCas9

Plasmid

#163535

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta3-3xFiBsd

Plasmid

#140962

Purpose

full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis associated 3 (Mta3 Mouse, Synthetic)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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