We narrowed to 14,262 results for: RON
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Plasmid#184959PurposeTest effect of a1/a2 length on editing fabH using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
ExpressionBacterialMutationfabH donor G954A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.mCherry-P2A-Post-SPHERE-SF-iGluSnFR
Plasmid#187102PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPost-SPHERE-SF-iGluSnFR
UseAAVTagsmCherry-P2AExpressionMammalianPromoterhSynAvailable SinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR
Plasmid#187101PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPre-SPHERE-SF-iGluSnFR
UseAAVTagsTagBFP-P2AExpressionMammalianPromoterhSynAvailable SinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- PGK1prom-TIR1-FLAG
Plasmid#189724PurposeEnables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)DepositorInsertOsTIR1
ExpressionYeastPromoterPGK1 promoterAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- TEF2prom-TIR1F74G-FLAG
Plasmid#189727PurposeEnables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)DepositorInsertOsTIR1
TagsFLAGExpressionYeastPromoterTEF2Available SinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS716
Plasmid#188888PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::CreDepositorInsertfloxed Cbr-unc-119 marker and Phsp16.41::Cre
ExpressionWormMutationC. elegans codom optimization and intron additionAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC3 smFP-HA-Cacna1e KI
Plasmid#183436PurposeCreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)DepositorInsertgRNA and smFP HA donor
UseCRISPRAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC5 Tubb3-GFP KI
Plasmid#183440PurposeFlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)DepositorInsertgRNA and GFP donor
UseCRISPRAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC2 Dlg4-Halo KI
Plasmid#183449PurposeCreOFF knock-in for PSD95-Halo (amino acid position: R721)DepositorInsertgRNA and Halo donor
UseCRISPRAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1: TfR1-CoilE
Plasmid#181988PurposeCoilE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with CoilR labeled with a reporterDepositorInsertTfR1-CoilE
TagsCoilEExpressionMammalianAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP221-AAV-RSV-GFP-pA
Plasmid#62919PurposeAAV vector backbone designed to express GFP from a RSV promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVPromoterRous Sarcoma Virus(RSV)Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
MmCavin4-APEX2-P2A-mKate2
Plasmid#170524PurposeVector for expression of MmCavin4-APEX2-P2A-mKate2 in mammalian cells. Parton lab clone EJFDepositorAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Tuba1b sgRNA / hSpCas9
Plasmid#172835PurposeMammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i1 sgRNA / hSpCas9
Plasmid#172825PurposeMammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_rSyn1 sgRNA / hSpCas9
Plasmid#172840PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_rActb sgRNA / hSpCas9
Plasmid#172833PurposeMammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-N1-MOB1
Plasmid#166477PurposeExpresses fusion of mCherry and MOB1DepositorInsertmCherry, MOB1 (MOB1A Human)
ExpressionMammalianAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGA
Plasmid#153530PurposeUsed as a donor vector for the targeted correction of a mutation in PIGA exon 6DepositorInsertPIGA (a 1,991-bp fragment from intron 5 and exon 6)
UseDonor plasmidTagsN/AMutationNo mutationPromoterNo promoterAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT5R1AtR2NtB2
Plasmid#160888PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T5
ExpressionBacterialPromoterrbcS-T5: T7, chaperones: T11Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK233 (AAVS1 CMV-AtAFB2)
Plasmid#140537PurposeAAVS1 CMV-AtAFB2DepositorInsertCMV-AtAFB2
ExpressionMammalianAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only