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pcDNA3-EKAR4

Plasmid

#174437

Purpose

Improved cyan/yellow FRET-based ERK kinase activity reporter.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

EKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pN1_Rev-GR-EGFP

Plasmid

#252750

Purpose

Plasmid for studying nuclear export. Expression of a fusion protein containing full-length HIV-1 Rev, followed by the hormone-responsive element of the rat glucocorticoid receptor (Gr), and GFP.

Depositor

Kristen Verhey

Article

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

HIV-1 Rev and Glucocorticoid Receptor (GR)

Tags

EGFP

Expression

Mammalian

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pSBbi-cpla2-mKate2-P2A-EGFP-KDEL

Plasmid

#250904

Purpose

Dual color expression of mKate2 tagged zebrafish cPla2 and ER markers (EGFP-KDEL) in mammallian cell lines

Depositor

Philipp Niethammer

Article

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

cPla2-mKate2-P2A-EGFP-KDEL (pla2g4aa Zebrafish)

Tags

KDEL on EGFP and mKate2 on cPla2

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pFA6a-yomRuby3-SpHIS5

Plasmid

#204287

Purpose

For C-terminal tagging using yeast optimized mRuby3

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pFA6-yomRuby3-SpHIS5

Tags

yomRuby3

Expression

Yeast

Promoter

none

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dPlcR

Plasmid

#181832

Purpose

Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMK444 (EF1-OsTIR1(F74G) Puro)

Plasmid

#214374

Purpose

EF1-OsTIR1(F74G) (PiggyBac)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

EF1-OsTIR1(F74G)

Expression

Mammalian

Promoter

EF1 promoter

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-H2BC11

Plasmid

#207755

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVHA E2F1

Plasmid

#24225

Purpose

Mammalian expression of human E2F1 with HA tag

Depositor

Kristian Helin

Article

Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Insert

E2F1 (E2F1 Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_mVenus

Plasmid

#113893

Purpose

Mammalian (inducible) expression of cytosolic mVenus

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

monomeric Venus

Use

Lentiviral

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep

Plasmid

#113883

Purpose

Mammalian (inducible) protein expression

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_mRuby2

Plasmid

#113894

Purpose

Mammalian (inducible) expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

monomeric Ruby2

Use

Lentiviral

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_EmGFP

Plasmid

#113892

Purpose

Mammalian (inducible) expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

Emerald GFP

Use

Lentiviral

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_mTurquoise2

Plasmid

#113895

Purpose

Mammalian (inducible) expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

monomeric Turquoise2

Use

Lentiviral

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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GPI-RpHLuorin2

Plasmid

#171721

Purpose

GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to GPI anchor

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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MS2-HB

Plasmid

#35573

Depositor

Marian Waterman

Article

Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

Insert

MS2 Coat Protein (cp Bacteriophage)

Use

Retroviral

Tags

HTBH

Expression

Mammalian

Mutation

Insert is a tail-to-head tandem placement of two …

Promoter

CMV

Available Since

May 30, 2012

Availability

Academic Institutions and Nonprofits only

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BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

Plasmid

#114595

Purpose

Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-NbALFA

Plasmid

#159987

Purpose

For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mCherry-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mCherry and NbALFA

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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hSyn1-DIO-mCherry-CAAX

Plasmid

#223673

Purpose

Cre-dependently expresses mCherry-CAAX in neurons

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mCherry-CAAX

Use

AAV and Cre/Lox

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pirespuro2-CTLA4-mEos2

Plasmid

#98285

Purpose

mammalian expression of CTLA4-mEos2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CTLA4 (CTLA4 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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UAS:mCherry

Plasmid

#170994

Purpose

UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

UAS:mCherry

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

Synthetic 14x UAS:Gal4

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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