Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 3,063 results for: ER

Clear keywords
Showing: 2051 - 2100 of 3063 results

  2. pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid
    #220610
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220610-plasmid-map-sequence-id-441133

    Enlarge

  4. pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

    Plasmid
    #220613
    Purpose
    Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only

  5. pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid
    #220614
    Purpose
    Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    Use
    AAV and Cre/Lox
    Tags
    3x HA; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220614-plasmid-map-sequence-id-441114

    Enlarge

  18. pETDuet-TFIIE

    Plasmid
    #171083
    Purpose
    Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    TF2E1 (GTF2E1 Human)
    TF2E2 (GTF2E2 Human)
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171083-plasmid-map-sequence-id-335640

    Enlarge

  22. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222497
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222497-plasmid-map-sequence-id-441134

    Enlarge

  23. pAAV-STAT-CA

    Plasmid
    #195576
    Purpose
    Expresses STAT3 constitutively active mutant
    Depositor
    Kevin Park
    Article
    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi
    Insert
    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    changed Alanine 662 to Cysteine and Asparagine 66…
    Promoter
    CMV enhancer and promoter
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195576-plasmid-map-sequence-id-383906

    Enlarge

  28. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

    Plasmid
    #185915
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185915-plasmid-map-sequence-id-367353

    Enlarge

  31. pAAV.hSynapsin.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106178
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    hSynapsin
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106178-plasmid-map-sequence-id-204208

    Enlarge

  32. pAAV.CAG.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106201
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    CAG
    Available Since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    106201-plasmid-map-sequence-id-201037

    Enlarge

  34. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106189
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    CAG
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106189-plasmid-map-sequence-id-204217

    Enlarge

  35. pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106177
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    hSynapsin
    Available Since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    106177-plasmid-map-sequence-id-204111

    Enlarge

  37. TRE3GV-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222502
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    TRE3GV and hPGK
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222502-plasmid-map-sequence-id-441221

    Enlarge

  43. pAAV.CAG.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106202
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    CAG
    Available Since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    106202-plasmid-map-sequence-id-204609

    Enlarge

  44. pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid
    #220608
    Purpose
    Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    Use
    AAV and Cre/Lox
    Tags
    3x HA; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220608-plasmid-map-sequence-id-441203

    Enlarge

  48. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid
    #197506
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197506-plasmid-map-sequence-id-386645

    Enlarge

  49. pAAV-syn-FLEX-axon-jYCaMP1s

    Plasmid
    #135421
    Purpose
    Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant
    Depositor
    Kaspar Podgorski
    Article
    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s
    Has Service
    AAV1
    Insert
    jYCaMP1s
    Use
    AAV
    Tags
    GAP43 axon targeting sequence
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Jan. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    135421-plasmid-map-sequence-id-268929

    Enlarge

Showing: 2051 - 2100 of 3063 results