Addgene

We narrowed to 1,122 results for: fret

Showing: 821 - 840 of 1122 results

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CaYin1 NES

Plasmid

#33318

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1 NES

Use

Tags

NES sequence LALKLAGLDIGS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PM-CaNARi

Plasmid

#64850

Purpose

Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

Lyn-CaNARi

Use

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-AktAR

Plasmid

#123356

Purpose

FRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

Lyn-AktAR

Use

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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TNXXL-CTS68

Plasmid

#48343

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

CTS68

Use

Tags

TN-XXL

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pDRF1-GW AKAR3-EV-NR

Plasmid

#182534

Purpose

Expresses the non responsive PKA FRET sensor AKAR3-EV (T506A) in budding yeast

Depositor

Bas Teusink

Article

Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

Insert

AKAR3-EV-NR

Use

Tags

Expression

Yeast

Mutation

T506A mutation

Promoter

PMA1

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pZac2.1 hSynapsin1 NAPA-N2 SV40

Plasmid

#97212

Purpose

FRET probe; extracelllular synapse targeted mScarlet

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

extracellular membrane tethered fluorescent protein

Use

AAV

Tags

Expression

Mutation

Promoter

hSynapsin1

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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CaYin1

Plasmid

#33067

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1

Use

Tags

6 His tag

Expression

Bacterial

Mutation

Promoter

ARA promoter

Available since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only

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274_pSKB3-Venus-LUMP

Plasmid

#65976

Purpose

Bacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensor

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Use

Tags

Venus and thrombin site (11-residue linker that h…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK-kinase0

Plasmid

#59122

Purpose

FAK fret control sensor without FERM domain with no linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK-FERM0

Plasmid

#59125

Purpose

FAK fret control sensor without kinase domain with no linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK-kinase10

Plasmid

#59123

Purpose

FAK fret control sensor without FERM domain with 10 nm linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 CMV NAPA-N BGH

Plasmid

#92278

Purpose

FRET probe; extracellular synapse targeted mCherry

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Neuronal

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK-FERM10

Plasmid

#59126

Purpose

FAK fret control sensor without kinase domain with 10 nm linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR221-Illusia

Plasmid

#215442

Purpose

Gateway entry vector with Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

none

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB.DEST-Illusia(YYFF)

Plasmid

#215445

Purpose

Piggybac expression vector with inactive phospho-mutant (YYFF) of Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(YYFF)

Use

Transposon-based stable expression

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-BLZinCh-P40

Plasmid

#188057

Purpose

Expression of BLZinCh-P40, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P40

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P50

Plasmid

#188058

Purpose

Expression of BLZinCh-P50, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P50

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P20

Plasmid

#188055

Purpose

Expression of BLZinCh-P20, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P20

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P30

Plasmid

#188056

Purpose

Expression of BLZinCh-P30, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P30

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-MTX_Snifit

Plasmid

#119043

Purpose

Plasmid encoding the bacterial expression of the control Snifit-MTX (FRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

Insert

MTX_Snifit

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R44L H45Q

Promoter

pBAD

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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