We narrowed to 881 results for: nanobody

1. GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)

   Plasmid

   #165028

   Purpose

   Express cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

   Depositor

   Yubin Zhou

   Article

   Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

   Insert

   LaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 5HT2A_NB2_1

   Plasmid

   #127690

   Purpose

   Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

   Depositor

   Bryan Roth, Justin English

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   5HT2A_NB2
   

   Expression

   Mammalian

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21b-LaG9(S151C)

   Plasmid

   #172490

   Purpose

   Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   LaG9(S151C)
   

   Tags

   6xHis, PPX cleavage site, and pelB leader sequenc…

   Expression

   Bacterial

   Mutation

   S151C

   Promoter

   T7

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR Ef1a_LaG16-synNotch_tTA-PGK_Puro

   Plasmid

   #223536

   Purpose

   Anti-GFP LaG16 nanobody-based synNotch; puro-selectable

   Depositor

   Jonathan Brunger

   Article

   Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

   Insert

   LaG16_synNotch_tTA
   

   Use

   Lentiviral

   Tags

   c-myc tag

   Expression

   Mammalian

   Available Since

   June 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-endo-Nb37

   Plasmid

   #205514

   Purpose

   Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-Nb37
   

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nb-gp41-GFP (MoonTag-Nb-GFP)

   Plasmid

   #128602

   Purpose

   Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   Nanobody_gp41
   

   Use

   Lentiviral

   Tags

   GB1 and sfGFP

   Promoter

   SFFV

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9 fused to scFv (anti-spike)

   Plasmid

   #186421

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-scFv fusion (anti-SARS-CoV-2 spike)
   

   Use

   CRISPR

   Tags

   6His

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVDL9.3

   Plasmid

   #168299

   Purpose

   Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

   Depositor

   Víctor de Lorenzo

   Article

   Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

   Insert

   Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
   

   Expression

   Bacterial

   Promoter

   LacI-Plac

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nb-gp41-Halo (MoonTag-Nb-Halo)

   Plasmid

   #128603

   Purpose

   Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   Nanobody_gp41
   

   Use

   Lentiviral

   Tags

   GB1 and Halo

   Promoter

   SFFV

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_886

    Plasmid

    #201162

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_887

    Plasmid

    #201164

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. APEX2-GBP

    Plasmid

    #67651

    Purpose

    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-endo-Nb80

    Plasmid

    #205518

    Purpose

    Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-Nb80
    

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-nuc-Nb37

    Plasmid

    #205515

    Purpose

    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb37
    

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA_888

    Plasmid

    #201165

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-nuc-Nb80

    Plasmid

    #205519

    Purpose

    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb80
    

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

    Plasmid

    #112157

    Purpose

    Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

    Depositor

    Ben Giepmans

    Article

    de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

    Insert

    anti-GFP FLIPPER-body
    

    Tags

    His and Thrombin cleavage site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmEos3.1-N1 GBP

    Plasmid

    #220492

    Purpose

    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos3.1 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

    Depositor

    Frederic Meunier

    Article

    Gormal et al Nat Protoc. 2025 Aug 8. doi: 10.1038/s41

    Insert

    GFP Binding Protein tagged with mEos3.1
    

    Tags

    mEos3.1

    Expression

    Mammalian

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7RNAPNev-NB1

    Plasmid

    #216336

    Purpose

    Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-eGFP nanobody NB1

    Depositor

    Mark Styczynski

    Article

    McSweeney et al Sci Adv. 2025 Feb 21;11(8):eado6280. doi

    Insert

    T7RNAPN-NB1 fusion
    

    Expression

    Bacterial

    Available Since

    July 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA3 JDM16-GFPnb-mCh

    Plasmid

    #235667

    Purpose

    Expresses designed inhibitor of Hsp70 tagged with a GFP nano body and mCherry

    Depositor

    David Baker

    Article

    Zhang et al Cell Chem Biol. 2025 Feb 4:S2451-9456(25

    Insert

    JDM16-GFPnb-mCh (Mch Synthetic)
    

    Tags

    GFP nanobody and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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