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KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB

Plasmid

#121837

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1

Plasmid

#121838

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR4-NLS

Plasmid

#138217

Purpose

Nuclear-targeted FRET-based PKA activity reporter.

Depositor

Jin Zhang

Publication

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AKAR4-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTrex-b-NLS-hSpCas9

Plasmid

#62543

Purpose

Expression of Cas9 in T. cruzi

Depositor

Rick Tarleton

Publication

Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS-hSpCas9

Use

CRISPR; Trypanosoma cruzi expression

Tags

3xFLAG and NLS

Promoter

Ribo-HX1

Available since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLD1112 TRE-NLS-mMaroon1-NLS-22Xm6A MS2

Plasmid

#235129

Purpose

mMaroon1 reporter plasmid for live cell visualization of RNA m6A modification

Depositor

Qin Peng

Publication

Li et al Sci Adv. 2024 Nov 15;10(46):eadp5689. do

Insert

TRE-NLS-mMaroon1-NLS-22Xm6A MS2

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-EGFP-NLS

Plasmid

#165441

Purpose

Expresses nuclear EGFP from CMV promoter

Depositor

Esteban Engel

Publication

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

EGFP-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-Nuc-ExRai-AMPKAR

Plasmid

#192450

Purpose

ExRai-AMPKAR targeted to the nucleus.

Depositor

Jin Zhang

Publication

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ExRai-AMPKAR-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLNCX myr Akt1

Plasmid

#17245

Depositor

Joan Brugge

Publication

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Akt1 (Akt1 Mouse)

Use

Retroviral

Tags

Myr

Expression

Mammalian

Mutation

Constitutively active

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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AAV_NLS-SaCas9-NLS-VPR

Plasmid

#68496

Purpose

AAV vector containing SaCas9 fused to VPR

Depositor

George Church

Publication

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

SaCas9-VPR

Use

AAV and CRISPR

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2WT_NLS

Plasmid

#221925

Purpose

Expression of SV40 nuclear localization signal (NLS) fused to optogenetic protein mCh-Cry2WT

Depositor

Daniel Bose

Publication

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

SV40 NLS

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

insertion of SV40 NLS

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSYC-103

Plasmid

#74791

Purpose

pCS4+-NLS-EGFP-E2A-mCherry-CAAX

Depositor

Seok-Yong Choi

Publication

Kim et al PLoS One. 2011 . 6(4):e18556.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS-EGFP-E2A-mCherry-CAAX

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-CNA35

Plasmid

#61607

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with mCherry

Depositor

Maarten Merkx

Publication

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mCherry and CNA35

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-CMVd1-NLS-GAL4-iLight2-VP16

Plasmid

#219550

Purpose

NLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Publication

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16

Use

AAV

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

Plasmid

#168290

Purpose

CRISPR-activator Suntag-P65-HSF1 (SPH)

Depositor

Hui Yang

Publication

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9, Suntag-P65-HSF1

Expression

Mammalian

Promoter

CAG

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-eBE-S3

Plasmid

#101093

Purpose

Expresses eBE-S3 in mammalian cells

Depositor

Jia Chen

Publication

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eBE-S3

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H151A; H1121A

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-eBFP2-NLS-pA

Plasmid

#183800

Purpose

AAV vector to express nuclear localized eBFP2 from CMV promoter

Depositor

Jonathan Ploski

Publication

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

eBFP2-NLS

Use

AAV

Tags

NLS

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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PIEZO1-HaloTag

Plasmid

#207834

Purpose

PIEZO1 fusion for biochemistry and live imaging

Depositor

Charles Cox

Publication

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PIEZO1 (PIEZO1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LEXY TR (without KRAB domain) (pDN162)

Plasmid

#72661

Purpose

LexATF-T2A-NLS-LexA-mCherry-LEXY construct for light control of transcriptional repression

Depositor

Barbara Di Ventura, Roland Eils

Publication

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LexATF-T2A-NLS-LexA-mCherry-LEXY

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD

Plasmid

#98669

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43

Depositor

Nicolas Fawzi

Publication

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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