We narrowed to 6,447 results for: nls

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pAAV-CMV-EGFP-NLS

Plasmid

#165441

Purpose

Expresses nuclear EGFP from CMV promoter

Depositor

Esteban Engel

Article

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

EGFP-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-ExRai-AMPKAR

Plasmid

#192450

Purpose

ExRai-AMPKAR targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2WT_NLS

Plasmid

#221925

Purpose

Expression of SV40 nuclear localization signal (NLS) fused to optogenetic protein mCh-Cry2WT

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

SV40 NLS

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

insertion of SV40 NLS

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSYC-103

Plasmid

#74791

Purpose

pCS4+-NLS-EGFP-E2A-mCherry-CAAX

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

NLS-EGFP-E2A-mCherry-CAAX

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pLNCX myr Akt1

Plasmid

#17245

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Akt1 (Akt1 Mouse)

Use

Retroviral

Tags

Myr

Expression

Mammalian

Mutation

Constitutively active

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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AAV_NLS-SaCas9-NLS-VPR

Plasmid

#68496

Purpose

AAV vector containing SaCas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

SaCas9-VPR

Use

AAV and CRISPR

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVd1-NLS-GAL4-iLight2-VP16

Plasmid

#219550

Purpose

NLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16

Use

AAV

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-eBE-S3

Plasmid

#101093

Purpose

Expresses eBE-S3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

eBE-S3

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-CNA35

Plasmid

#61607

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with mCherry

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mCherry and CNA35

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

Plasmid

#168290

Purpose

CRISPR-activator Suntag-P65-HSF1 (SPH)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

dCas9, Suntag-P65-HSF1

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-eBFP2-NLS-pA

Plasmid

#183800

Purpose

AAV vector to express nuclear localized eBFP2 from CMV promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

eBFP2-NLS

Use

AAV

Tags

NLS

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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LEXY TR (without KRAB domain) (pDN162)

Plasmid

#72661

Purpose

LexATF-T2A-NLS-LexA-mCherry-LEXY construct for light control of transcriptional repression

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

LexATF-T2A-NLS-LexA-mCherry-LEXY

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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PIEZO1-HaloTag

Plasmid

#207834

Purpose

PIEZO1 fusion for biochemistry and live imaging

Depositor

Charles Cox

Article

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

PIEZO1 (PIEZO1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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dxCas(3.6)-VPR

Plasmid

#108388

Purpose

Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

dxCas(3.6)-VPR

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD

Plasmid

#98669

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

Plasmid

#81206

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR2-NLS

Plasmid

#175012

Purpose

Nuclear-targeted AktAR2.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

AktAR-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4

Plasmid

#219711

Purpose

Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.

Depositor

Jennifer Nemhauser

Insert

GG0-pAHP6:PhiC31-NLS:tUBQ1-GG4

Use

Synthetic Biology

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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MTK4a_003

Plasmid

#123817

Purpose

Encodes Nuclear import sequence as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NLS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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