-
Plasmid#24403DepositorInsertBeclin1 (BECN1 Human)
UseTagsHisExpressionBacterialMutationContains the CC domain (aa 151-242)PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHC1_ARATH
Plasmid#204237PurposeBacterial expression of Arabidopsis cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHC1_ARATH (AT1G04410 Mustard Weed)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PaODH
Plasmid#110802PurposeExpresses PaODH and opine dehydrogenase from Pseudomonas aeruginosaDepositorInsertPa4835
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15TV-L-SaNAS
Plasmid#110803PurposeExpresses SaNAS a nicotianamine synthase from Staphylcoccus aureusDepositorInsertSAV2469 (SAV2469 )
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PaNAS
Plasmid#110801PurposeExpresses PaNAS nicotianamine synthase from pseudomonas aeruginosaDepositorInsertPa4836
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15TV-L-SaODH
Plasmid#110804PurposeExpresses SaODH and opine dehydrogenase from Staphylococcus aureusDepositorInsertSAV2468 (SAV2468 )
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(cd226)
Plasmid#177900PurposeBacterial expression of his10-tagged human cd226 Intracellular DomainDepositorInsertCD226 (aa 288-336 only) (CD226 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PSAT
Plasmid#74115PurposeExpression of PSATDepositorInsertpET28a-PSAT (PSAT1 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CLTC_Clathrin propeller
Plasmid#178471PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCLTC_Clathrin propeller (CLTC Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 TIM8,13
Plasmid#170282PurposeExpresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim8 and Tim13 (TIM8 Budding Yeast)
UseTagsTEV-cleavable N-terminal His tag on Tim13 (on bac…ExpressionBacterialMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
yPet_cp229-Binder
Plasmid#179137PurposeYPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).DepositorInsertSspB
UseTagsyPet_cp229ExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9PromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-Npl4
Plasmid#21267DepositorInsertNpl4 (Nploc4 Rat)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-N
Plasmid#30225DepositorInsertrhVitronectin with N terminus cleavage (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 20*-62PromoterAvailable sinceMay 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-HsPABPC1_J
Plasmid#146642PurposeBacterial Expression of HsPABPC1DepositorInsertHsPABPC1 (PABPC1 Human)
UseTagsExpressionBacterialMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-pETM30
Plasmid#85768Purposebacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusionDepositorInsertUSPL1, catalytic domain (aa 212-514) (USPL1 Human)
UseTagsGST and HisExpressionBacterialMutationcatalytic domain (aa 212-514)PromoterT7-lacAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET3a-Ub-D77
Plasmid#18896DepositorInsertUbiquitin-D77 (UBC Human)
UseTagsExpressionBacterialMutationAspartate residue is added to the C-terminus of t…PromoterAvailable sinceSept. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Rx3(A7)
Plasmid#35525DepositorInsertHis6-Rx3(A7)
UseTagsHis6ExpressionBacterialMutationChanged G2 to PPromoterT7Available sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED19
Plasmid#15430DepositorInsertMED19 (Med19 Mouse)
UseTagsGST and HisExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET23 P53DBD
Plasmid#174503Purposeexpress P53 DNA binding domain (94-312) in E.coli without tagDepositorInsertP53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluh
Plasmid#119830PurposeProduction of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluh
UseTagsGSTExpressionBacterialMutationE25APromoterT7Available sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only