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Plasmid#97125Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmicroRNA 218a 2 Dendra2
UseZebrafishTagsExpressionMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-microRNA Let 7-2-dendra2
Plasmid#97103Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmicroRNA Let 7 2 dendra2
UseZebrafishTagsExpressionMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-microRNA 19c-Dendra2
Plasmid#97104Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmicroRNA 19c Dendra2
UseZebrafishTagsExpressionMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-microRNA 22b-Dendra2
Plasmid#97105Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmicroRNA 22b Dendra2
UseZebrafishTagsExpressionMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-NONHSAT073641
Plasmid#92432Purposemammalian expression of lncRNA NONHSAT073641DepositorInsertNONHSAT073641
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
ARK1a
Plasmid#81016PurposeE.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-PMKco
PMD-NudB
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pInducer20
Plasmid#44012PurposeTet-inducible lentiviral vector for ORF expressionDepositorTypeEmpty backboneUseLentiviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-N346A/C348A
Plasmid#127411PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethersDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationN346A-C348APromoteraraBADAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
SB100X in pCAG globin pA
Plasmid#127909PurposeSleeping Beauty Transposase expression vector driven by the CAG promoterDepositorInsertSleeping Beauty Transposase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
4xHRE-MinTK-CRE-ODD
Plasmid#141147Purposelentiviral expression vector to generate a hypoxia fate-mapping systemDepositorInsert4xHRE-MinTK-CRE-ODD
UseLentiviralTagsExpressionMutationcontains an altered Cre gene modified by the addi…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-loxp-DsRed-loxp-eGFP
Plasmid#141148Purposelentiviral expression vector to generate a hypoxia fate-mapping systemDepositorInsertloxp-DsRed-loxp-eGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLR16-Phes*
Plasmid#98783PurposeSuicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).DepositorInsertpheS
UseTagsExpressionBacterialMutationA309GPromoterAvailable sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC101_TIMER
Plasmid#103057Purposelow copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)DepositorInsertTIMER-bac
UseTagsExpressionBacterialMutationmutations: S4T and S179TPromoterconstitutive ybaJp promoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPEPY-PF6-lacI
Plasmid#85589PurposeWork as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniaeDepositorInsertlacI
UseTagsExpressionBacterialMutationPromoterPF6Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJWV102-PL-dCas9
Plasmid#85588PurposeIntegrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9spDepositorInsertdCas9sp
UseCRISPRTagsExpressionBacterialMutationPromoterPLspAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCmEos3.2:cat
Plasmid#78100PurposePlasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.DepositorInsertmEos3.2-cat
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
dCas9 fused to nanobody (anti-beta catenin)
Plasmid#186420PurposeExpression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tagDepositorInsertdCas9-nanobody fusion (anti-beta catenin)
UseCRISPRTags6HisExpressionMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRedCm
Plasmid#176225PurposeLambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phageDepositorInsertLambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaB and PLAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
sIL6R PLAK1
Plasmid#161519PurposeExpresses soluble IL6 receptor in mammalian cells.DepositorInsertsoluble IL-6 receptor
UseLentiviralTags3X FlagExpressionMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-TET-GFP-FF3
Plasmid#11662Purpose3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferaseDepositorInsertFF3
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only