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We narrowed to 5,233 results for: phy

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Showing: 821 - 840 of 5233 results

  1. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  2. pASKt15C+SrtA5

    Plasmid
    #64985
    Purpose
    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, D160N, D165A, K19…
    Promoter
    tetracycline promoter/operator
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64985-plasmid-map-sequence-id-109954

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  6. MSP2266

    Plasmid
    #70705
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70705-plasmid-map-sequence-id-131909

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  9. pTd-4-15-13-10

    Plasmid
    #63835
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available Since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. p305N-5

    Plasmid
    #246288
    Purpose
    Evaluation of AtU6.1m2 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.1m2 promoter
    Use
    CRISPR
    Expression
    Plant
    Mutation
    A-to-C (-63 from TSS) and G-to-T (-57) within USE
    Promoter
    AtU6.1m2 promoter
    Available Since
    Dec. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    246288-plasmid-map-sequence-id-494268

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  15. p305N

    Plasmid
    #246316
    Purpose
    A promoterless construct designed for cloning various Pol III promoters or serving as a control in evaluating Pol III promoters for CRISPR-Cas9 editing of mEGFP in Nicotiana benthamiana and poplar reporter lines
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    Promoterless
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Promoterless
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246316-plasmid-map-sequence-id-491208

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  16. p305N-2

    Plasmid
    #246285
    Purpose
    Evaluation of AtU3d promoter (Pol III promoter) for CRISPR-Cas9 editing of mEGFP in Nicotiana benthamiana and poplar reporter lines
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU3d promoter
    Use
    CRISPR
    Expression
    Plant
    Promoter
    AtU3d promoter
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246285-plasmid-map-sequence-id-491187

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  18. p305N-8

    Plasmid
    #246291
    Purpose
    Evaluation of AtU6.29c14 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.29c14 promoter
    Use
    CRISPR
    Expression
    Plant
    Mutation
    T-to-C (-1 from TSS)
    Promoter
    AtU6.29c14 promoter
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246291-plasmid-map-sequence-id-491184

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  19. p305N-6

    Plasmid
    #246289
    Purpose
    Evaluation of AtU6.1m3 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.1m3 promoter
    Use
    CRISPR
    Expression
    Plant
    Mutation
    G-to-T (-57 from TSS) within USE
    Promoter
    AtU6.1m3 promoter
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246289-plasmid-map-sequence-id-491182

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Showing: 821 - 840 of 5233 results