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Plasmid#212156PurposePlasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.DepositorInsertsecondary alcohol dehydrogenase
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHFdist_rm
Plasmid#162318PurposeP. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removedDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationIHF distal site is removedPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBZS-YP_001498439
Plasmid#198370PurposeExpress YP_001498439 in bacterial cells.DepositorInsertYP_001498439
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
ptRNAfMet-A37U
Plasmid#196659PurposePlasmid for overexpression of mutated initiator tRNA (A37U)DepositorInserttRNAfMet-A37U expression cassette
UseTagsExpressionBacterialMutationA37U in tRNAfMetPromotermetY native promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
ptRNAfMet-A37G
Plasmid#196658PurposePlasmid for overexpression of mutated initiator tRNA (A37G)DepositorInserttRNAfMet-A37G expression cassette
UseTagsExpressionBacterialMutationA37G in tRNAfMetPromotermetY native promoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENT L1-Kozak-ERK-KTR-L2
Plasmid#186353PurposeEntry clone with ORF encoding ERK KTR flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertERK Kinase Translocation Reporter (MAPK1 Synthetic, Human)
UseProtein expressionTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-luc-miR-124
Plasmid#171558Purposea positive control reporter with 3' UTR sequences perfectly complementary to miR-124DepositorInserta sequences perfectly complementary to miR-124
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-luc-miR-1
Plasmid#171556Purposea positive control reporter with 3' UTR sequences perfectly complementary to miR-1DepositorInserta sequences perfectly complementary to miR-1
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
C238-E07: attB2r-SV40polyA-attB3
Plasmid#162917PurposeGateway attB2r/attB3 entry clone containing a polyA sequence for transcription terminationDepositorInsertSV40 polyadenylation sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC_cherry donor_Rosa target (pE002)
Plasmid#149348PurposeReplace donor vector for cherry insertion using the mouse Rosa26 guide RNADepositorInsertcherry
UseTagsExpressionMutationPromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC_cherry donor_Polb target (pE038)
Plasmid#149349PurposeReplace donor vector for cherry insertion using the human Polb guide RNADepositorInsertcherry
UseTagsExpressionMutationPromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE22
Plasmid#161787PurposeHi-SC2 reporter delivery plasmidDepositorInsertHi-SC2 reporter
UseConjugative plasmid to deliver hi-sc2 reporterTagsExpressionMutationHimar site included MmeI site, tandem loxP sites,…Promotern/aAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH298
Plasmid#115651Purposerecombination substrateDepositorInsertV(D)J recombination substrate
UseTagsExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-MMP
Plasmid#112632PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-MMP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB2629
Plasmid#113394PurposeMET25p-MS2-RFP-CYC1 TTDepositorInsertMS2 bacteriophage coat protein
UseTagsred fluorescent protein (RFP), codon-optimized fo…ExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK_proNR2_LGK.9
Plasmid#81162PurposeExpresses levoglucosan kinase variant LGK.9 with promoter proNR2DepositorInsertLevoglucosan kinase
UseTagsHis6xExpressionBacterialMutationLGK.9 - (T15R, A57Q, R94G, R100C, N101G, S105R, L…PromoterproNR2Available sinceSept. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVD518
Plasmid#66158PurposeSix copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probeDepositorInserttdh1
UseCloning vectorTagsExpressionMutationPromoterUnknownAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 to gap
Plasmid#49158PurposeTemplate for generating gene 60 mRNA, sequence 5' of coding gap onlyDepositorInsertBacteriophage T4 gene 60
UseTagsExpressionMutationCoding gap and downstream sequence have been del…PromoterT7Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-MYO5C
Plasmid#135404PurposeExpresses GFP-tagged human MYO5C in mammalian cells.DepositorInsertMYO5C (MYO5C Human)
UseTagsEGFPExpressionMammalianMutationRelative to the GenBank MYO5C cDNA sequence repor…PromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIG-601_NY-ESO-1_TCR_GFP
Plasmid#207482PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertGFP, NY-ESO-1_TCR
UseCRISPRTagsExpressionMutationPotential silent point mutation in GFP sequencePromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only