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Plasmid#47108PurposeExpresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertSPgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1
Plasmid#194278PurposeExpresses gRNA and dSaCas9-KRAB from lentiviral vectorDepositorInsertshumanized dSaCas9 KRAB T2A Thy1.1
gRNA
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhU6 and hUbCAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP
Plasmid#71237PurposeExpress sgRNA and dCas9-KRAB from 3rd generation lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A GFP
sgRNA
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75154PurposeNuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP
Plasmid#92388PurposeCell death reporterDepositorInsertANNEXINV (ANXA5 Human)
UseZebrafish expressionTagsmVenusExpressionMutationPromoterubbAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9
Plasmid#47106PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cellsDepositorInsertdCas9
UseCRISPRTagsFlag and SV40 NLSExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWMD23
Plasmid#140006PurposeExpression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)DepositorInsertsORF1 Shuffle 1 NLS
Pong TPase LA
UseTagsExpressionPlantMutationNES sequence mutated L418A, L420A and Ping N-term…PromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-mpeg1.1
Plasmid#75023PurposeCan be used to drive expression in zebrafish microglia.DepositorInsertmacrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromotermpeg1.1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-R-GECO1.2
Plasmid#45494PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2DepositorInsertR-GECO1.2
UseTagsExpressionMammalianMutationR-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDRM98 mKate2 Blast
Plasmid#174720PurposeExpresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.DepositorInsertsmKate2
BSD
UseLentiviralTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianMutationPromoterMND and MND (off mKate-P2A)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry
Plasmid#71282PurposeZebrafish expression vector for heat-shock inducible expression of APEX2-GBPDepositorInsertAPEX2-GBP
UseZebrafish expression with tol2TagsExpressionMutationPromoterHSP70lAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-OSBP
Plasmid#67664PurposeMammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-OSBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EB3mCherry_cmv-LifeActGFP
Plasmid#133927PurposeLive dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeletonDepositorInsertEB3-mCherry and Lifeact-GFP (MAPRE3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate2-KDEL-pA
Plasmid#109546PurposeMultisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)DepositorInsertmKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
CDK1as_T2A_puromycin
Plasmid#118595PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-NEUROD1-T2A-PuroR
Plasmid#162338PurposeLentiviral expression of NEUROD1 under the control of the TetON promoterDepositorInsertNEUROD1 (NEUROD1 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pR2Q024y
Plasmid#101022Purposefluorescent, functional, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
UseTagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromotercmVAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-gRNA-UbC-DsRed-P2A-Bsr
Plasmid#83919PurposeLentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastRDepositorInsertsDsRed-Express2
Bsr
UseCRISPR and LentiviralTagsP2AExpressionMutationPromoterhUbCAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMVd1-EGFP-hRhoA-wt
Plasmid#133926PurposeLow level expression of GFP fused human RhoA wild typeDepositorInsertGFP-hRhoA WT (RHOA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only