We narrowed to 28,446 results for: REP

1. TRAIP-SFB

   Plasmid

   #101769

   Purpose

   Mammalian expression of TRAIP fusion protein

   Depositor

   Michael Huen

   Article

   Feng et al Cell Discov. 2016 Jun 28;2:16016. doi: 1

   Insert

   TRAIP / RNF206 (TRAIP Human)
   

   Use

   Tags

   S peptide-flag-streptavidin binding peptide (SFB)

   Expression

   Mammalian

   Mutation

   siRNA-resistant allele

   Promoter

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAP24

   Plasmid

   #70190

   Purpose

   Modular vector for secreted luciferase reporter expression

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

   Insert

   sLucopt
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSN444

   Plasmid

   #108331

   Purpose

   Expresses Sat2 for Nourseothricin resistance selection of C. elegans

   Depositor

   Shinsuke Niwa

   Article

   Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

   Insert

   Streptothricin acetyl transferase 2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rps-27 promoter

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOCC154

   Plasmid

   #118902

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Use

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOCC153

   Plasmid

   #118901

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKB01_mOrange2

   Plasmid

   #69102

   Purpose

   Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   mOrange2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 tdAPX W41F

   Plasmid

   #40863

   Purpose

   Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

   Insert

   tandem dimer pea APX W41F
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   W41F mutation

   Promoter

   CMV

   Available since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. HUW-TetO-hNanog

   Plasmid

   #25700

   Depositor

   Rudolf Jaenisch

   Article

   Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

   Insert

   Nanog (NANOG Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

   Plasmid

   #133339

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_ctrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJES103

    Plasmid

    #135410

    Purpose

    E. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporter

    Depositor

    Miriam Braunstein

    Article

    McCann et al Microbiology. 2007 Oct;153(Pt 10):3350-9

    Insert

    blaTEM-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E. coli 'blaTEM-1 with M. tuberculosis mpt63…

    Promoter

    hsp60

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. psiCHECK-2-Zeb2-3'UTR

    Plasmid

    #61771

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Zeb2

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Zeb2 3'UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

12. KA2628_pGL4-UBCmin-luc2/Tbx3-B'

    Plasmid

    #124201

    Purpose

    Luciferase reporter driven by the Tbx3 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Tbx3 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTU1-A-SP44-mVenus-I

    Plasmid

    #163763

    Purpose

    Expresses Streptomyces codon-optimised mVenus-I

    Depositor

    Simon Moore, Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Insert

    mVenus-I
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    SP44

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJFRC7-UAS-NPRR_ANP

    Plasmid

    #133243

    Purpose

    Fluorescent reporter for neuropeptide release imaging

    Depositor

    David Anderson

    Article

    Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

    Insert

    rat atrial natriuretic peptide-fused codon-optimized GCaMP6s
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pY2

    Plasmid

    #218115

    Purpose

    Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMaCTag-P29

    Plasmid

    #120040

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Strep-II
    

    Use

    Pcr template

    Tags

    Strep-II

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. [Bla][IRES][GFP]

    Plasmid

    #97193

    Purpose

    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Inserts

    beta-Lactamase
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAcGFP-Jpx9C104T

    Plasmid

    #120381

    Purpose

    Reporter for RNA nuclear localization

    Depositor

    Igor Ulitsky

    Article

    Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

    Insert

    JPX (JPX Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    C321T

    Promoter

    CMV

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG306-pTet07-synYFP[TTG/AGA]-nLuc

    Plasmid

    #199756

    Purpose

    Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-nLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Donor_eGP2AP_RC

    Plasmid

    #133784

    Purpose

    Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

    Depositor

    Sriram Kosuri

    Article

    Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

    Type

    Empty backbone

    Use

    Tags

    eGFP-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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