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rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRES-GFP

Plasmid

#32477

Purpose

Cre-dependent expression of PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F-5HT3HC

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available Since

Jan. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN beta

Plasmid

#40929

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN beta (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

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CAV1-K*R-mEGFP

Plasmid

#27766

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-flex-iGABASnFR2n-WPRE

Plasmid

#218878

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

Synapsin

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC H-Ras V12

Plasmid

#18741

Depositor

Scott Lowe

Article

Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

Insert

H-Ras V12 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

V12

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha

Plasmid

#40928

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32475

Purpose

Cre-dependent expression of PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G-5HT3HC

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available Since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pMJ923

Plasmid

#78313

Purpose

Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

Depositor

Martin Jinek

Article

Burger et al Development. 2016 Apr 29. pii: dev.13480

Insert

SpCas9

Tags

HA-2xNLS-mCherry-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

human codon-optimized gene sequence

Promoter

T7

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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hCD12

Plasmid

#225541

Purpose

Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

Depositor

David Christianson

Article

Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

Insert

Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)

Tags

His6-MBP tag with cleavable TEV site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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N-Myc-53BP1 D1521A pLPC-Puro

Plasmid

#19837

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

p53 Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

Myc

Mutation

changed Asp1521 to Ala

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

Plasmid

#66789

Purpose

Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Article

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRalpha (Pdgfra Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

D842V (constitutively active)

Promoter

EF1a

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only
pLenti CMV TRE3G BCL2L11 Neo

Plasmid

#140747

Purpose

Inducibly expresses BIM in mammalian cells

Depositor

Scott Dixon

Article

Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

Insert

BCL2L11 (BCL2L11 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV TRE3G

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMW96

Plasmid

#178066

Purpose

Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

Depositor

Tarun Kapoor

Article

Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

Insert

CDK5RAP2 (CDK5RAP2 Human)

Expression

Bacterial

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-FLIM-AKART391A

Plasmid

#60446

Purpose

Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKART391A

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

changed Threonine 391 to Ananine

Promoter

CAG

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-flex-iGABASnFR2(no bind)-WPRE

Plasmid

#218882

Purpose

AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2(no bind)

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

S99A F102G R168P

Promoter

CAG

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-flex-lck-smV5

Plasmid

#196423

Purpose

Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificity

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

Lck-smV5

Use

AAV and Cre/Lox

Tags

Lck

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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LP455

Plasmid

#90198

Purpose

pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 649-1426

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

NPHP4 (NPHP4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

aa residues 649-1426

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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LP453

Plasmid

#90184

Purpose

pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 1-839

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

NPHP4 (NPHP4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

aa residues 1-839

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV Myc P57S IRES GFP

Plasmid

#18771

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

myc P57S (MYC Human)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

P57S

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
KCC2B_HUMAN_D0

Plasmid

#79718

Purpose

This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2B

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only