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Showing: 8461 - 8480 of 9093 results

  3. pMDC32B-BS-AtMIR390a-A18G-B/c

    Plasmid
    #246716
    Purpose
    Cloning amiRNA sequences in AtMIR390a-A18G precursors for in planta expression
    Depositor
    Alberto Carbonell
    Article
    Llorens-Gamez et al Plant J. 2026 Jan;125(1):e70665. doi: 10
    Insert
    Basal stem of Arabidopsis MIR390a precursor with A18G mutation & chloramphenicol-ccdB cassette flanked by two BsaI sites (MIR390a Mustard Weed)
    Use
    RNAi
    Expression
    Plant
    Available Since
    Jan. 14, 2026
    Availability
    Academic Institutions and Nonprofits only
    246716-plasmid-map-sequence-id-494273

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  4. pEvolvR-mCherry-eNme2.C.NR-nCas9(D16A)-PolI5MΔ

    Plasmid
    #249077
    Purpose
    Expresses nucleus-localized eNme2.C.NR-nCas9 (D16A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    eNme2.C.NR-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249077-plasmid-map-sequence-id-496316

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  5. pREC1010-opEas1

    Plasmid
    #240701
    Purpose
    RSF1010 origin of replication plasmid containing Eas1 recombitron with extended a1 a2 regions in the ncRNA targeting lacZ locus in Klebsiella pneumoniae ATCC 10031 expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103
    Insert
    Eas1 RT, Eas1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    Extended a1 a2 regions
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    240701-plasmid-map-sequence-id-489548

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  6. RC00214-pcsb-35Sp-ccdB-mNG-OCSp-tdT

    Plasmid
    #209019
    Purpose
    Dual-fluorescence acceptor plasmid for one-step insertion of a 5' leader of interest between the CaMV35S promoter and mNeonGreen via BpiI. A tdTomato expression cassette serves as internal control.
    Depositor
    Stephen Long
    Article
    Haas et al Plant Direct. 2026 Apr 15;10(4):e70163.
    Inserts
    ccdB
    mNeonGreen
    tdTomato
    Expression
    Plant
    Mutation
    codon optimized for N. benthamiana
    Promoter
    CaMV35S and Octopine Synthase
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    209019-plasmid-map-sequence-id-461176

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  8. pREC1010-opEbu1

    Plasmid
    #240702
    Purpose
    RSF1010 origin of replication plasmid containing Ebu1 recombitron with extended a1 a2 regions with a donor in the ncRNA targeting lacZ locus in Citrobacter freundii ATCC 8090 expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103
    Insert
    Ebu1 RT, Ebu1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    Extended a1 a2 regions
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240702-plasmid-map-sequence-id-482374

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  13. KL095 - pTRE3G-Flag-Halo-CTCF_ZF1_mutant-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #232931
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF ZF1 mutant, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    Flag-Halo-CTCF ZF1 deletion mutant
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    Deleted amino acids 264-277
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232931-plasmid-map-sequence-id-466490

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  15. pdnCas3-CDA1

    Plasmid
    #229535
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229535-plasmid-map-sequence-id-459013

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  16. pdnCas3-APOBEC1

    Plasmid
    #229537
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229537-plasmid-map-sequence-id-459005

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  19. AA320

    Plasmid
    #215952
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0 [EnAs]; sgCD47_v1 [EnAs]; DR_v1 [EnAs]; sgCD47_v2 [EnAs]}
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215952-plasmid-map-sequence-id-431483

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  20. AA315

    Plasmid
    #215951
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215951-plasmid-map-sequence-id-431482

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Showing: 8461 - 8480 of 9093 results