We narrowed to 9,085 results for: mel

1. pAcUW51-gE2

   Plasmid

   #11621

   Depositor

   Pamela Bjorkman

   Article

   Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

   Insert

   HSV-1 glycoprotein E
   

   Tags

   His

   Expression

   Insect

   Mutation

   deleted amino acids 391-550

   Available Since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA5-FRT-TetO-fSNAPc

   Plasmid

   #106986

   Purpose

   open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   fSNAP
   

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. GGDestX1-Amp

   Plasmid

   #157652

   Purpose

   Golden Gate assembly and cell-free (bacterial) expression of fusion-proteins

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Cell-free expression (e. coli sigma-70)

   Expression

   Bacterial

   Promoter

   OR2-OR1-Pr

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPS2192

   Plasmid

   #8923

   Depositor

   Pamela Silver

   Article

   Hood et al J Cell Sci 2001 Feb;114(Pt 3):589-97.

   Insert

   Clb2-deltaNLS-GFP (CLB2 Budding Yeast)
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Deletion of CLB2 bipartite NLS (aa 183-200), KpnI…

   Available Since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK504

   Plasmid

   #155381

   Purpose

   dCas12a crRNAs

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Insert

   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAH3

   Plasmid

   #41827

   Purpose

   Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.

   Depositor

   Melissa Moore

   Article

   Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

   Type

   Empty backbone

   Use

   Pcr template

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. MKT074

   Plasmid

   #231997

   Purpose

   MicF RNA toehold switch with NanoLuc complementation peptide reporter

   Depositor

   Melissa Takahashi

   Article

   Diaz et al MicroPubl Biol. 2025 Jan 2;2025:10.17912

   Insert

   MicF RNA toehold switch
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. avi-eTMPRSS11D S368A

   Plasmid

   #220882

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TMPRSS11D (TMPRSS11D Human)
   

   Use

   Baculovirus expresssion

   Tags

   Honeybee melittinsignal

   Mutation

   S368A

   Promoter

   Polyhedrin

   Available Since

   June 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLenti puro HA-Ubiquitin

   Plasmid

   #74218

   Purpose

   Lentiviral expression of HA tagged Ubiquitin

   Depositor

   Melina Fan

   Insert

   ubiquitin (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Single ubiquitin coding sequence (full length UBB…

   Promoter

   CMV

   Available Since

   March 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pTIPI_mIGg2a_Anti-HA [12CA5]

    Plasmid

    #210656

    Purpose

    Mammalian expression of Anti-HA [12CA5] mouse IgG2a heavy chain; to be used with pTIPI_mK_Anti-HA [12CA5] light chain (Plasmid 210657) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-HA [12CA5] mouse IgG2a heavy chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Mouse IgG2a heavy chain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pTIPI_mK_Anti-HA [12CA5]

    Plasmid

    #210657

    Purpose

    Mammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-HA [12CA5] kappa light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Mouse kappa light chain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA_mIGg2a_Anti-HA [12CA5] v2

    Plasmid

    #236295

    Purpose

    Mammalian vector expressing Anti-HA [12CA5] variable region fused to mouse IgG2a heavy chain; to be used with pcDNA_mK_Anti-HA [12CA5] v2 light chain (Plasmid 236296) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-HA [12CA5] heavy chain variable region fused to mouse IgG2a heavy chain
    

    Tags

    Mouse IgG2a Fc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pcDNA_mK_Anti-HA [12CA5] v2

    Plasmid

    #236296

    Purpose

    Mammalian vector expressing Anti-HA [12CA5] variable region fused to mouse Kappa light chain; to be used with pcDNA_mIGg2a_Anti-HA [12CA5] v2 heavy chain (Plasmid 236295) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-HA [12CA5] light chain variable region fused to mouse kappa light chain
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGEMHE-NLS-mEGFP

    Plasmid

    #105527

    Purpose

    T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Nucleoplasmin
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Mutation

    NLS of nucleoplasmin (encoding amino acids KRPAAT…

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1D V5-His-TOPO-HuR.wt

    Plasmid

    #110417

    Purpose

    Mammalian expression of HuR (wild type)

    Depositor

    Sandra Martha Gomes Dias

    Insert

    HuR (ELAVL1) (ELAVL1 Human)
    

    Tags

    6xHis and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-myc-Wnk1

    Plasmid

    #38779

    Depositor

    Melanie Cobb

    Article

    Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

    Insert

    Wnk1 (Wnk1 Rat)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Tet-inducible- FLAG-Core R (pMO30)

    Plasmid

    #24479

    Depositor

    Melanie Ott

    Article

    Schwer et al J Virol. 2004 Aug . 78(15):7958-68.

    Insert

    Core R
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

18. PAS528 (TJF059)

    Plasmid

    #65602

    Purpose

    pETDuet-1 Containing FadD-His

    Depositor

    Pamela Silver

    Article

    Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

    Insert

    FadD
    

    Tags

    6x His

    Expression

    Bacterial

    Promoter

    T7 lacO

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pWS1806

    Plasmid

    #123047

    Purpose

    Melatonin GPCR

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    MTNR1A [3]
    

    Expression

    Yeast

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKM263-ScPPX

    Plasmid

    #38327

    Depositor

    Florian Freimoser

    Article

    Werner et al Arch Microbiol. 2005 Nov;184(2):129-36.

    Insert

    PPX1 (PPX1 Budding Yeast)
    

    Tags

    GST, His, and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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