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We narrowed to 63,694 results for: cloning

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Showing: 8501 - 8520 of 63694 results

  2. pcDNA5-FRT-TOgw-C-mEGFP

    Plasmid
    #223953
    Purpose
    Gateway cloning of genes of interest with a C-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mEGFP
    Expression
    Mammalian
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223953-plasmid-map-sequence-id-450835

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  3. pcDNA5-FRT-TOgw-C-mECFP

    Plasmid
    #223950
    Purpose
    Gateway cloning of genes of interest with a C-terminal mECFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mECFP
    Expression
    Mammalian
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223950-plasmid-map-sequence-id-450833

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  4. pcDNA5-FRT-TOgw-C-mEYFP

    Plasmid
    #223951
    Purpose
    Gateway cloning of genes of interest with a C-terminal mEYFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mEYFP
    Expression
    Mammalian
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223951-plasmid-map-sequence-id-450832

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  5. pcDNA5-FRT-TOgw-N-mEGFP

    Plasmid
    #223949
    Purpose
    Gateway cloning of genes of interest with an N-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mEGFP
    Expression
    Mammalian
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223949-plasmid-map-sequence-id-450830

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  6. pJC226

    Plasmid
    #198637
    Purpose
    MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system
    Depositor
    Shaun Curtin
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    June 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    198637-plasmid-map-sequence-id-394770

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  7. pKP579

    Plasmid
    #159598
    Purpose
    Express Cba and Cbi protein
    Depositor
    Kathleen Postle
    Insert
    cba and cbi
    Expression
    Bacterial
    Mutation
    none
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    159598-plasmid-map-sequence-id-313311

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  8. pGEX6P1-DEST-Myc

    Plasmid
    #119752
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and Myc
    Expression
    Bacterial
    Promoter
    tac promoter
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    119752-plasmid-map-sequence-id-233674

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  9. R777-E112 Hs.IRS2-nostop

    Plasmid
    #70396
    Purpose
    Gateway ORF clone of codon optimized human IRS2 [NM_003749] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    IRS2 (IRS2 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70396-plasmid-map-sequence-id-125845

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  14. pFGL815

    Plasmid
    #52322
    Purpose
    A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.
    Depositor
    Naweed Naqvi
    Publication
    Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10
    Type
    Empty backbone
    Use
    Agrobacterium
    Expression
    Bacterial
    Available since
    July 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52322-plasmid-map-sequence-id-81025

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  16. BB3cH_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104912
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104912-plasmid-map-sequence-id-206066

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  19. R777-E279 Hs.RHOB

    Plasmid
    #70563
    Purpose
    Gateway ORF clone of human RHOB [NM_004040.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RHOB (RHOB Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70563-plasmid-map-sequence-id-126012

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  20. R777-E236 Hs.RASAL2-nostop

    Plasmid
    #70520
    Purpose
    Gateway ORF clone of human RASAL2 [NM_170692.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASAL2 (RASAL2 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70520-plasmid-map-sequence-id-125969

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Showing: 8501 - 8520 of 63694 results