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pJMP2774

Plasmid

#160667

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Publication

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Expression

Bacterial

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCCl-MCS_miniCMV_TagBFP

Plasmid

#134986

Purpose

The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Publication

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

TagBFP

Use

Lentiviral

Promoter

minCMV

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pKL08

Plasmid

#104432

Purpose

pHuji in yeast expression vector

Depositor

Joel Kralj

Publication

Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

Insert

pHuji

Expression

Yeast

Promoter

Tef1

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-APIC-Cas9

Plasmid

#121657

Purpose

gateway cloning destination vector for expressing enhancer driven Cas9

Depositor

Chun Han

Publication

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pJMP1069

Plasmid

#119244

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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NK690

Plasmid

#120849

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Jumble-mWasabi

Use

Choanoflagellate expression

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pJF131B

Plasmid

#113326

Purpose

pTet-W108 scRNA.b2, pTet-RR2-sgRNA

Depositor

Jesse Zalatan

Publication

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

W108 scRNA_MS2 and sgRNA_RR2

Use

CRISPR

Expression

Bacterial

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.719

Plasmid

#108520

Purpose

PTtg-YFP Reporter. Nar sensor for use in Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PTtg-YFP

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.711

Plasmid

#108512

Purpose

PPhlF-YFP Reporter. DAPG sensor for use in Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PPhlF-YFP

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBBR6k-J23109-GFPuv

Plasmid

#106392

Purpose

pBBR6k-GFPuv with Anderson promoter J23109 expressing lacI

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

LlacO-1

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBBR6k-J23101-GFPuv

Plasmid

#106390

Purpose

pBBR6k-GFPuv with Anderson promoter J23101 expressing lacI

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

LlacO-1

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMulti-sgRNA-LacZ

Plasmid

#99913

Purpose

Receptor plasmid for the assembly of multiple sgRNAs

Depositor

Yujie Sun

Publication

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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ZIKV_NASBA_32B

Plasmid

#75011

Purpose

Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

Depositor

James Collins, Alexander Green

Publication

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_NASBA_32B

Expression

Bacterial

Promoter

T7

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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ZIKV_NASBA_27B

Plasmid

#75010

Purpose

Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

Depositor

James Collins, Alexander Green

Publication

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_NASBA_27B

Expression

Bacterial

Promoter

T7

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBN280

Plasmid

#86731

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Ptph-1::FLP D5

Expression

Worm

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBN266

Plasmid

#86732

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pdpy-7::FLP D5

Expression

Worm

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBS5

Plasmid

#83799

Purpose

Useful for tagging yeast proteins with N-terminal CFP tag; G418 selection

Depositor

Eric Muller

Publication

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

CFP

Expression

Yeast

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO SV40

Plasmid

#68455

Purpose

TOPO construct expressing SV40 for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Simian Virus Early 40 Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pKS012

Plasmid

#65465

Purpose

pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherry

Depositor

Keith Tyo

Publication

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

Ntag-mCherry

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

pl-TetO

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pTHSSd_1

Plasmid

#59947

Purpose

Splitposon in KanR ColE1 backbone

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

Splitposon w/ KanR, LacI & PTac

Expression

Bacterial

Mutation

N/A

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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