We narrowed to 26,711 results for: Cat

1. lentiCRISPR - DDX3Y sgRNA 1

   Plasmid

   #70656

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against DDX3Y
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. VYAIFIFQL_epitope

   Plasmid

   #165018

   Purpose

   Expression of CMV US20 (29-37) fused to IL-2 signal sequence

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   CMV US20 (29-37)
   

   Use

   Lentiviral

   Tags

   human IL2 signal sequence at N-term

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4046764

   Plasmid

   #145673

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. CROP_C9_Puro_PDGFRB

   Plasmid

   #183314

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets PDGFRB gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   PDGFRB
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMC-Ura-Cen-lvl1

   Plasmid

   #176182

   Purpose

   preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. coreR1 (384-1008)

   Plasmid

   #115646

   Purpose

   expression of truncated Rag1

   Depositor

   Martin Gellert

   Article

   Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

   Insert

   Rag1 core (384-1008) (Rag1 Mouse)
   

   Use

   Tags

   8XHis-MBP-PreScission

   Expression

   Mammalian

   Mutation

   truncated Rag1 (aa 384-1008)

   Promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. aq-CFP

   Plasmid

   #36073

   Depositor

   Narasimhan Gautam

   Article

   Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

   Insert

   G protein subunit alpha q (Gnaq Mouse)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   CFP inserted between amino acid 124 and 125

   Promoter

   CMV

   Available since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pH6HTC_YTHDF1-Halo

   Plasmid

   #175301

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   YTHDF1 (YTHDF1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 AT1R DRY-AAY

   Plasmid

   #45759

   Depositor

   Robert Lefkowitz, Laszlo Hunyady

   Article

   Gaborik et al Endocrinology. 2003 Jun;144(6):2220-8.

   Insert

   AT1R DRY-AAY (Agtr1a Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed amino acids 125-126 from DR to AA

   Promoter

   CMV

   Available since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCMB-TGN61r

    Plasmid

    #61175

    Purpose

    Fluorescent reporter for trans-Golgi network mCherry-MtSYP61 expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtSYP61
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHiUGE Myc-epitope donor ORF0

    Plasmid

    #200374

    Purpose

    Tagging endogenously expressed proteins with Myc epitope, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    Myc epitope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHiUGE Myc-epitope donor ORF1

    Plasmid

    #200375

    Purpose

    Tagging endogenously expressed proteins with Myc epitope, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    Myc epitope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH_ss-SBP-EGFP

    Plasmid

    #172358

    Purpose

    Reporter to monitoring a streptavidin-binding peptide trafficking

    Depositor

    Guido Kroemer

    Article

    Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

    Insert

    ER importing signal sequence
    

    Use

    Lentiviral

    Tags

    SBP-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET14_GFP5_Encap

    Plasmid

    #86408

    Purpose

    Expresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operon

    Depositor

    David Savage

    Article

    Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

    Inserts

    sfGFP with a 5aa CLP tag
    Encapsulin
    

    Use

    Tags

    6x Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.1-Tmem98-FLAG

    Plasmid

    #124501

    Purpose

    Expresses FLAG-tagged mouse TMEM98 in mammalian cells (C-terminal tag)

    Depositor

    Renee van Amerongen

    Article

    van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

    Insert

    Tmem98 (Tmem98 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBW-m4092686

    Plasmid

    #153895

    Purpose

    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nearly native encoding;inactivating mutations in …

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pEBB-BT-Ubiquitin

    Plasmid

    #36098

    Depositor

    Ezra Burstein

    Article

    Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

    Insert

    Ubiquitin (RPS27A Human)
    

    Use

    Tags

    TB-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. NAX_CAGGS_CAS9_EYFP

    Plasmid

    #167858

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. BAT-lux

    Plasmid

    #20890

    Depositor

    Stefano Piccolo

    Article

    Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

    Insert

    7xTCF binding sites, Siamois minimal promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    7 multimerized TCF-binding elements fused upstrea…

    Promoter

    Available since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pT2-cryR;clecl14aCE1-P1Egfp

    Plasmid

    #90157

    Purpose

    contains clec14a enhancer sequence, and clec14a specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    clec14aE1P1Egfp
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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