We narrowed to 16,584 results for: EMB

1. p3E-V5-mScarlet-I_SV40pA (JDW 968)

   Plasmid

   #224529

   Purpose

   A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   V5-mScarlet-I
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEasyCas9

   Plasmid

   #184909

   Purpose

   Expresses the Streptococcus pyogenes Cas9 protein

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMMK ENTR 2

   Plasmid

   #206261

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Type

   Empty backbone

   Use

   Multimate/gateway entr 2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyTag003-OspC

   Plasmid

   #159998

   Purpose

   Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag003-OspC (20-209) C129S
   

   Use

   Tags

   His6-tag and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   C129S mutation improves expression level.

   Promoter

   T7

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS_FLPER(T2)

   Plasmid

   #31303

   Depositor

   Gerhart Ryffel

   Article

   Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

   Insert

   FLP-ER ligand binding domain (ESR1 Human, Budding Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   full length FLP linked to ligand binding domain o…

   Promoter

   Available since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLuciferase (GB0096)

   Plasmid

   #68201

   Purpose

   Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Luciferase coding region
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPyCAG-EGFP-TM-IRES-Pac

   Plasmid

   #183603

   Purpose

   Mammalian expression vector for membrane-tethered EGFP from the CAG promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   EGFP-TM
   

   Use

   Tags

   Human PDGFRB transmembrane domain and Mouse IgGK …

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGK:HygroR-CMV:mTagBFP2-A_UTR

   Plasmid

   #68479

   Purpose

   Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Hygromycin Resistance
   mTagBFP2-A_UTR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   shRNA sensor cassette "A" added to 3&#x…

   Promoter

   CMV and pGK

   Available since

   Nov. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mRIP3 GFP RHIM mut

   Plasmid

   #41384

   Depositor

   Francis Chan

   Article

   Cho et al Cell. 2009 Jun 12;137(6):1112-23.

   Insert

   RIP3 (Ripk3 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   RHIM mutant

   Promoter

   CMV

   Available since

   Dec. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

    Plasmid

    #86414

    Purpose

    nuclear split GFP11 reporter without mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-GFP11-PUS1
    

    Use

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    NOP1pr

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCX-Klf4

    Plasmid

    #66656

    Purpose

    For expression of mouse Klf4.

    Depositor

    Barak Cohen

    Article

    Fiore et al Genome Res. 2016 Jun;26(6):778-86. doi:

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1-pDUXCca

    Plasmid

    #198236

    Purpose

    Lentivector with tet-inducible porcine DUXC (codon altered)

    Depositor

    Stephen Tapscott

    Article

    Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

    Insert

    porcine DUXC (codon altered)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon altered

    Promoter

    TRE

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-InPAkt

    Plasmid

    #181914

    Purpose

    Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

    Insert

    InPAkt
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AA345

    Plasmid

    #216031

    Purpose

    Fragmid fragment: (Cas protein) Cas13d

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas13d_v1.1 [Dj]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-FKBP-GFP-OPTN (2-119)

    Plasmid

    #208867

    Purpose

    Stably express GFP-tagged OPTN (2-119aa) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    OPTN (OPTN Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    aa 2-119 only

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-kcnk3a

    Plasmid

    #208985

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk3a (kcnk3a Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRDA_834

    Plasmid

    #216087

    Purpose

    Cas9 [Sp] knockout targeting CD59, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD59 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. YFP-DAXX-mSIM1

    Plasmid

    #134524

    Purpose

    Expresses YFP-DAXX fusion protein with a mutation in its 5' SIM

    Depositor

    Susan Janicki

    Article

    Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

    Insert

    DAXX-mSIM1
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    SIM (IIVL) 7-10 aa changed to IGAG

    Promoter

    CMV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKB01-PssbB-mKate2

    Plasmid

    #69105

    Purpose

    Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mKate2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGS1222

    Plasmid

    #63791

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eGFP-Twin-Strep tag followed by URA3
    

    Use

    E. coli cloning vector

    Tags

    eGFP-Twin-Strep tag

    Expression

    Mutation

    Promoter

    none

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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