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pENTR221-SMAD3

Plasmid

#79507

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

SMAD3 (SMAD3 Human)

Use

Gateway Cloning

Tags

FLAG

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTX168

Plasmid

#89257

Purpose

Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

Depositor

Daniel Voytas

Publication

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMpGWB300

Plasmid

#68628

Purpose

MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB100

Plasmid

#68062

Purpose

MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-GFP

Plasmid

#75130

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Publication

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDGB3_alpha2R

Plasmid

#68231

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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E1010m (RFP)_CD

Plasmid

#66033

Purpose

MoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Fluorescent reporter - RFP

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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R0010 (pLacI)_AB

Plasmid

#66004

Purpose

MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pES002 (pTet-qacR wt)

Plasmid

#69031

Purpose

wild-type qacR downstream of a Tet promoter

Depositor

Richard Murray

Publication

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Tags

His6 tag

Expression

Bacterial

Promoter

pTet

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b-Fab1 (Francisella tularensis)

Plasmid

#61690

Purpose

Inducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensis

Depositor

Michael Johnson

Publication

Hevener et al J Med Chem. 2012 Jan 12;55(1):268-79. do

Insert

enoyl-[acyl-carrier-protein] reductase (NADH)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only
mEos2-LaminB1-10

Plasmid

#57395

Purpose

Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

LaminB1 (LMNB1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-TER+-shLKB1-Ms

Plasmid

#61234

Purpose

Entry clone encoding shRNA to mouse LKB1

Depositor

Reuben Shaw

Publication

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (STK11, Stk11 Human, Mouse)

Use

Entr clone

Promoter

H1/TO

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pHL 1405

Plasmid

#53010

Purpose

pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp

Depositor

Han Lim

Publication

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

RhyB sRNA
DsrA sRNA
hfq
sodB

Use

Synthetic Biology

Tags

gfp

Expression

Bacterial

Mutation

contains -56 to +141 relative to start codon

Promoter

pCon, pLlacO-1, and pLtetO-1

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pVRa38_1322

Plasmid

#49698

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF38_1322

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro-TRF2 deltaB

Plasmid

#18010

Depositor

Titia de Lange

Publication

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Deleted Basic Domain (aa1-44)

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
PL2

Plasmid

#12395

Depositor

David Baker

Publication

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

PL2

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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pBS/U6/hTID1

Plasmid

#13745

Depositor

Karl Munger

Publication

Edwards et al Oncogene. 2004 Nov 4. 23(52):8419-31.

Insert

hTid1 siRNA (DNAJA3 Human)

Use

RNAi

Mutation

Inverted repeat of 21 bp with 6 nuc. spacer.

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA4-C-EF1α

Plasmid

#239097

Purpose

Empty pcDNA4-C backbone with EF1α promoter.

Depositor

David Cobrinik

Publication

Shayler et al eLife 13:RP101918

Type

Empty backbone

Expression

Mammalian

Promoter

EF1α

Available since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKD013

Plasmid

#207931

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M, for braiding

Depositor

Alexis Maizel

Publication

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMP108

Plasmid

#207928

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M:FastRed, for braiding

Depositor

Alexis Maizel

Publication

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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