We narrowed to 9,182 results for: Pol

1. pEIAV-DIO-TLoop-CHR2YFP

   Plasmid

   #66798

   Purpose

   expression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vector

   Depositor

   Edward Callaway

   Article

   Cetin et al J Neurophysiol. 2014 May;111(10):2150-9.

   Insert

   Floxed Chr2-YFP
   

   Use

   Lentiviral

   Tags

   citrine

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CMVTetO7 Promoter

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPV-H1-ccdB-mEF1α-RiH

   Plasmid

   #100598

   Purpose

   piggyBac vector for sgRNA cloning

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Type

   Empty backbone

   Use

   Piggybac vector

   Expression

   Mammalian

   Promoter

   Human H1 PolIII promoter, human FTH1 promoter, mo…

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DCT.VV.Orf170

   Plasmid

   #12858

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf170
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pSCT-GAL93-SFPQ

   Plasmid

   #46320

   Depositor

   Steven Brown

   Article

   Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

   Insert

   SFPQ (SFPQ Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pFastBacDual with LFn + Needle

   Plasmid

   #84868

   Purpose

   Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Needle
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUDE1093

   Plasmid

   #204227

   Purpose

   Episomal yeast plasmid expressing the Ercas12a

   Depositor

   Jean-Marc Daran

   Article

   Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

   Insert

   Eubacterium rectale cas12a (Ercas12a)
   

   Expression

   Yeast

   Mutation

   codon optimized for S. cerevisiae

   Promoter

   Saccharomyces cerevisiae PGK1

   Available Since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP787_SnoopCatcher-EZH2-FL-CO

   Plasmid

   #211780

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-DNMT3A
   

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBacDual with LFn + Fla

   Plasmid

   #84866

   Purpose

   Baculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Fla
   

   Use

   Baculovirus

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMuLE ENTR 7SK-miR-30 L1-L4

   Plasmid

   #62180

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   RNAi; Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

10. p3E-V5-mScarlet-I_SV40pA (JDW 968)

    Plasmid

    #224529

    Purpose

    A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mScarlet-I
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOCC119

    Plasmid

    #118884

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease and mGFP, cl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-LacI-HIPK2

    Plasmid

    #179269

    Purpose

    Expresses GFP-LacI-HIPK2 fusion protein

    Depositor

    Lienhard Schmitz

    Article

    Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

    Insert

    homeodomain interacting protein kinase 2 (HIPK2 Human)
    

    Tags

    GFP-LacI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-hPPP3R1

    Plasmid

    #179136

    Purpose

    Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TIA1 RRM-YFP-SspBx6

    Plasmid

    #103781

    Purpose

    expresses iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    TIA1 RRM-YFP-SspBx6
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFL-EGFP-3xFlag-Fml1

    Plasmid

    #193058

    Purpose

    Baculovirus expression of S.pombe Fml1 for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    Fml1 (fml1 Fission Yeast)
    

    Tags

    3xFlag

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-MSP1D1deltaH4/2H5

    Plasmid

    #71715

    Purpose

    Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ATF4 7: 5’3’ATF4.GFP

    Plasmid

    #21854

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Vector derived viral termination and poly-adenyla…

    Available Since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-MSP1D1deltaH4/2

    Plasmid

    #71712

    Purpose

    Membrane scaffold protein (MSP), half of helix 4 truncated

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. px330 RBP1 gRNA

    Plasmid

    #165593

    Purpose

    Insertion of RBP1 degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    RBP1 (Polr2a Mouse)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AV44_pCAG.Cas9-D10A.gRNA.S1

    Plasmid

    #199258

    Purpose

    Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9-D10A nickase
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge