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YAP1-V5 in pLX304

Plasmid

#42555

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(wt)--2A-mCherry-puro

Plasmid

#231974

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Mutation

WT

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pPD0055_pIVTRup_MS2-p65-HSF1

Plasmid

#242541

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

MCP-p65-HSF1 (RELA, HSF1 Human)

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(1-180)-2A-mCherry-puro

Plasmid

#231976

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL 1-180 construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_CreNLS_PP7

Plasmid

#232439

Purpose

expression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTR

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

Cre–MYC-NLS

Expression

Mammalian

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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elavl3:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218852

Purpose

D. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Tags

QF fusion protein

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-CBP core

Plasmid

#179543

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-IDH1

Plasmid

#62906

Purpose

Expresses wildtype IDH1 in mammalian cells

Depositor

Yue Xiong

Article

Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

Insert

isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)

Tags

Flag

Expression

Mammalian

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pNK2657

Plasmid

#219741

Purpose

MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

hispidin- synthase from Mycena citricolor

Use

Synthetic Biology

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9Pubi-H

Plasmid

#66187

Purpose

Expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

maize ubiquitin promoter (Pubi)

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSYC-437

Plasmid

#163829

Purpose

Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

Depositor

Seok-Yong Choi

Article

Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

Inserts

Foxj1a Promoter
GCaMP6s

Use

Multisite gateway destination vector

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hATG5 E122D

Plasmid

#111804

Purpose

Mammalian Expression of hATG5

Depositor

Jun Hee Lee

Article

Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

Insert

hATG5 (ATG5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

E122D

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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evoFERNY-Cas9NG

Plasmid

#203329

Purpose

Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

evoFERNY-Cas9NG

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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Ai65(RCFL-tdT) targeting vector

Plasmid

#61577

Purpose

Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

pCAG-FSF-LSL-tdTomato

Use

Cre/Lox and Mouse Targeting

Promoter

CAG

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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anti-GFP scFv [N86/38] with sortase tag

Plasmid

#204419

Purpose

Mammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting GFP (Aequorea victoria)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

Plasmid

#162377

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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miABE

Plasmid

#205412

Purpose

Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

Insert

pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR

Expression

Mammalian

Mutation

D61A+E85R+H369R+S387R+S457R

Promoter

hU6, CAG, CMV

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TP53-KO_gRNA_1

Plasmid

#195130

Purpose

TP53-targeting gRNA in the LRG2.1 backbone

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

TP53 KO gRNA 1 (TP53 Human)

Expression

Mammalian

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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shYAP1 # 1

Plasmid

#42540

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV_Cas9–superNLS

Plasmid

#232430

Purpose

Expression plasmid for the SpCas9 nuclease with superNLS

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

Cas9–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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