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We narrowed to 69,707 results for: nin

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Showing: 8561 - 8580 of 69707 results

  1. pCMV-T7-SpCas9(KRHWMR)-P2A-EGFP_(RAS3808)

    Plasmid
    #228251
    Purpose
    pCMV human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRHWMR)-P2A-EGFP
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(ARGIMR)=D1135K, S1136R, G1218H, E1219W, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    228251-plasmid-map-sequence-id-451023

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  2. pMT1219

    Plasmid
    #228301
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variant
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His (S Synthetic, Severe acute respiratory syndrome-related coronavirus 2)
    Use
    Synthetic Biology
    Tags
    GGG linker followed by His6 and MF_ secretion sig…
    Expression
    Yeast
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228301-plasmid-map-sequence-id-457688

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  3. pCMVtrunc msfGFPΔC10-Utr28-222

    Plasmid
    #231558
    Purpose
    Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Utrophin calponin homology domain (UTRN Human)
    Tags
    msfGFP
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231558-plasmid-map-sequence-id-459698

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  4. Anti-Kv2.1 K+ channel [K89/34R-rat IgG2a]

    Plasmid
    #225417
    Purpose
    Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (Rat) rat IgG2a R-mAb. Derived from hybridoma K89/34.
    Depositor
    James Trimmer
    Article
    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1
    Insert
    Anti-Kv2.1 K+ channel (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Kcnb1 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225417-plasmid-map-sequence-id-448492

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  5. Anti-Kv4.2 K+ channel (external) [K57/1R-rat IgG2a]

    Plasmid
    #225419
    Purpose
    Mammalian Expression Plasmid of anti-Kv4.2 K+ channel (external) (Human) rat IgG2a R-mAb. Derived from hybridoma K57/1.
    Depositor
    James Trimmer
    Article
    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1
    Insert
    Anti-Kv4.2 K+ channel (external) (Homo sapiens) recombinant (Mouse) monoclonal antibody. (KCND2 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225419-plasmid-map-sequence-id-448489

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  6. Anti-Mortalin [N52A/42R-rat IgG2a]

    Plasmid
    #225423
    Purpose
    Mammalian Expression Plasmid of anti-Mortalin (Mouse) rat IgG2a R-mAb. Derived from hybridoma N52A/42.
    Depositor
    James Trimmer
    Article
    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1
    Insert
    Anti-Mortalin (Mus musculus) recombinant (Mouse) monoclonal antibody. (Hspa9 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225423-plasmid-map-sequence-id-448485

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  11. pT3-EF1A-MYC-IRES-lucOS

    Plasmid
    #129776
    Purpose
    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339
    Depositor
    Amaia Lujambio
    Article
    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82
    Insert
    human MYC (MYC Human)
    Tags
    OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    129776-plasmid-map-sequence-id-263368

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  14. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #156432
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    none
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156432-plasmid-map-sequence-id-310156

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  16. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid
    #223127
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223127-plasmid-map-sequence-id-447695

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  18. pSLIK-NFLAG-hEWSR1G511A-CMyc

    Plasmid
    #50734
    Purpose
    Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 G511A (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Mutation
    Changed Gly 511 to Ala
    Promoter
    TRE
    Available Since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50734-plasmid-map-sequence-id-481096

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  19. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  20. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

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Showing: 8561 - 8580 of 69707 results