We narrowed to 2,412 results for: organization Addgene

1. pMTTH+eGFP-bromo5(N140A)

   Plasmid

   #157806

   Purpose

   Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   eGFP-bromo5(N140A)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   N140A

   Promoter

   pTAC

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-LifeAct-FRB-Venus-173-N

   Plasmid

   #87616

   Purpose

   FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   LifeAct-FRB-Venus173-N
   

   Use

   Tags

   17-aa sequence from Abp140 (aka "Lifeact&quo…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKI-cjFOXL2-coemiRFP670-PGK-Puro

   Plasmid

   #186168

   Purpose

   Knock-in gene targeting in marmoset cells at FOXL2 locus

   Depositor

   Sho Yoshimatsu

   Article

   Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

   Insert

   emiRFP670
   

   Use

   Marmoset targeting

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6_T7_INT[6xminimal_Puf1]

   Plasmid

   #107608

   Purpose

   Transient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoter

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   6x minimal Puf1 motif
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. H6-epsin 1 (144-575)

   Plasmid

   #63008

   Purpose

   expresses the adaptor protein epsin that lacks its ENTH-domain in bacteria

   Depositor

   Ernst Ungewickell

   Article

   Kalthoff et al J Biol Chem. 2002 Mar 8;277(10):8209-16.

   Insert

   Epsin 1 without ENTH domain (Epn1 Rat)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2015

   Availability

   Academic Institutions and Nonprofits only

6. mCherry-miniSOG-Peroxisomes-2

   Plasmid

   #54804

   Purpose

   Localization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Type

   Empty backbone

   Use

   Tags

   Peroximal Targeting Signal 1 (PTS1) and mCherry-m…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

7. 5mCUC1 pGreenII0129

   Plasmid

   #12067

   Depositor

   David Bartel

   Article

   Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

   Insert

   CUC1 mutant (CUC1 Mustard Weed)
   

   Use

   Plant transformation via agrobacterium

   Tags

   Expression

   Mutation

   miR-resistant CUC1. Made by introducing five sile…

   Promoter

   Available since

   June 2, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pCDNA6/ERGIC2-WT

   Plasmid

   #51479

   Purpose

   Expresses ERGIC2-WT in mammalian cells

   Depositor

   Simon Kwok

   Article

   Kwok et al DNA Cell Biol. 2001 Jun;20(6):349-57.

   Insert

   ERGIC2-WT (ERGIC2 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   March 6, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCS2 DN-xIGFR

   Plasmid

   #24699

   Depositor

   Edward De Robertis

   Article

   Pera et al Mech Dev. 2002 Aug . 116(1-2):169-72.

   Insert

   Dominant negative xIGFR (igf1r Frog)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C-terminal truncated version of the Xenopus IGF t…

   Promoter

   Available since

   June 7, 2010

   Availability

   Academic Institutions and Nonprofits only

10. PA-mCherry-miniSOG-Lifeact-7

    Plasmid

    #54846

    Purpose

    Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    PA-mCherry-miniSOG-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only