We narrowed to 3,920 results for: pCMV mammalian

1. pCMV4a-SIRT2-Flag

   Plasmid

   #102623

   Purpose

   Expresses full length SIRT2 isoform 1 in mammalian cells

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   Sirtuin 2 (SIRT2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV4a-Flag-c-Myc

   Plasmid

   #102625

   Purpose

   Expresses c-Myc in mammalian cells

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV5-Flag-XBP1s

   Plasmid

   #63680

   Purpose

   Mammalian expression of Flag tagged XBP1s

   Depositor

   Lingfang Zeng, Qingbo Xu

   Article

   Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

   Insert

   XBP1 (XBP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   spliced

   Promoter

   CMV

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6-AN-HA_WT RAD23A

   Plasmid

   #201458

   Purpose

   Expresses human RAD23A with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Myc-HNF4A

   Plasmid

   #219396

   Purpose

   Express HNF4A in mammalian cells

   Depositor

   Yoichi Furukawa

   Article

   Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

   Insert

   HNF4A (HNF4A Human)
   

   Use

   Tags

   N terminal Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV 3xFLAG-UBR5

   Plasmid

   #231238

   Purpose

   Flag-tagged wild-type UBR5 over-expression construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   UBR5 (UBR5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6-AN-HA_WT POLH

   Plasmid

   #201671

   Purpose

   Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   Polymerase eta (POLH Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcmv3-IRG1-N152S-HA

   Plasmid

   #198182

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1) N152S

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The asparagine in IRG1 at position 152 mutates to…

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcmv3-IRG1-L272Q-HA

   Plasmid

   #198184

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1) L272Q

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The leucine in IRG1 at position 272 mutates to gl…

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-DeAct-SpvB

    Plasmid

    #89446

    Purpose

    Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-SpvB (spvB Salmonella enterica)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation spanning SpvB amino acids 375-591 (mon…

    Promoter

    CMV

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-dCas9-D3A

    Plasmid

    #78256

    Purpose

    Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter

    Depositor

    Grant Challen

    Article

    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

    Insert

    DNMT3A CD aa 598-912 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_T7-PE2-Nuclease

    Plasmid

    #171997

    Purpose

    Sequence provided for PE-Nuclease mRNA transcription, suitable for microinjection

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CMV_T7-Cas9-RT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GC to CA point mutation in SpCas9 to restore nucl…

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6-AN-DDK_WT POLI

    Plasmid

    #131228

    Purpose

    Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Coding sequence has been codon optimised for expr…

    Promoter

    CMV enhancer + CMV promoter

    Available since

    Dec. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV3Tag8li-hMBOAT7-3xFLAG

    Plasmid

    #165474

    Purpose

    Human MBOAT7 mammalian expression vector with 3 C-terminal FLAG tags.

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    Membrane bound O-acyltransferase domain containing 7 (MBOAT7 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-N2-6MYC-hMID2

    Plasmid

    #51035

    Purpose

    mammalian expression of myc-tagged human MID2

    Depositor

    Timothy Cox

    Article

    Short et al BMC Cell Biol. 2002;3:1. Epub 2002 Jan 4

    Insert

    MID2 (MID2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV6-AN-DDK_UBM1*2* POLI

    Plasmid

    #131232

    Purpose

    Mammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domains

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains inactivating mutations in both ubiquitin…

    Promoter

    CMV enhancer + CMV promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-BE3-FNLS(RA)

    Plasmid

    #112671

    Purpose

    CMV expression vector for BE3-FNLS construct (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3-FNLS
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    CMV

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-mGold-Mito-N-7

    Plasmid

    #158007

    Purpose

    Visualization of mitochondria in mammalian cells using mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-hAR-CA576-Z

    Plasmid

    #89108

    Purpose

    mammalian expression of full-length human androgen receptor with DNA binding domain mutation CA576

    Depositor

    Elizabeth Wilson

    Article

    Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

    Insert

    hAR-C576A (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C576A

    Promoter

    CMV

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-mGold-MannII-N-10

    Plasmid

    #158005

    Purpose

    Visualization of Golgi in mammalian cells using mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    MannII (Man2a1 Mouse)
    

    Use

    Tags

    mGold

    Expression

    Mammalian

    Mutation

    Insert contains only aa 1-112 of MannII

    Promoter

    CMV

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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