We narrowed to 4,504 results for: pcr

1. pBALU-ext

   Plasmid

   #69339

   Purpose

   It allows to extend the genomic insert in a fosmid.

   Depositor

   Oliver Hobert

   Article

   Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

   Insert

   kanamycin gene +pCC1FOS homology
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pAEF6

   Plasmid

   #136303

   Purpose

   pAEF5 plasmid with two gRNA inserted

   Depositor

   Gilles Fischer

   Article

   Agier et al Methods Mol Biol. 2021;2196:181-198. doi

   Insert

   double cassette coding for gRNA
   

   Use

   CRISPR; Template for pcr to insert double grna co…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BB1_L_23_syn_BsaI

   Plasmid

   #89915

   Purpose

   Linker to construct BB1 from PCR product with FS2 and FS3 (in with BsaI, out with BbsI)

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LBJJ383

   Plasmid

   #117517

   Purpose

   PCR template for sgRNA classic with polyT as terminator

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. L0-I38 BpiI-pUBQ10-BpiI

   Plasmid

   #195888

   Purpose

   Level 0 pUBQ10 promoter between BpiI sites for target construction

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   pUBQ10
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. L0-T4 tRBSC_mScarlet

   Plasmid

   #195887

   Purpose

   Level 0 tRBSC-mScarlet for integrase target construction

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   tRBCS-mScarlet
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENTR-D-TOPO-kcnt2b

   Plasmid

   #164964

   Purpose

   Zebrafish kcnt2a gene CDS

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

   Insert

   kcnt2b (LOC565691 Zebrafish)
   

   Use

   Pcr cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BB1_L_12_syn_BsaI

   Plasmid

   #89914

   Purpose

   Linker to construct BB1 from PCR product with FS1 and FS2 (in with BsaI, out with BbsI)

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BB1_L_34_syn_BsaI

   Plasmid

   #89916

   Purpose

   Linker to construct BB1 from PCR product with FS3 and FS4 (in with BsaI, out with BbsI)

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pECT1B

    Plasmid

    #75447

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pECT1D

    Plasmid

    #75448

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pECT1E

    Plasmid

    #75449

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TAL-2CC3

    Plasmid

    #41485

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    2CC3
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

14. TAL-4AA1

    Plasmid

    #41496

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    4AA1
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

15. TAL-4GC1

    Plasmid

    #41505

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    4GC1
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

16. TAL-4TC1

    Plasmid

    #41509

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    4TC1
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

17. TAL-1CA2

    Plasmid

    #41448

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    1CA2
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

18. TAL-1CT2

    Plasmid

    #41451

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    1CT2
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

19. TAL-1GT2

    Plasmid

    #41455

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    1GT2
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

20. TAL-1TA2

    Plasmid

    #41456

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    1TA2
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

21. TAL-1TT2

    Plasmid

    #41459

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    1TT2
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

22. TAL-3AA4

    Plasmid

    #41460

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    3AA4
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

23. TAL-3AC4

    Plasmid

    #41461

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    3AC4
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

24. TAL-3GA4

    Plasmid

    #41468

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    3GA4
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pPOTv7-blast-10HA

    Plasmid

    #181941

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    10xHA
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYZ038

    Plasmid

    #200227

    Purpose

    universal template to build tRNA-gRNA array for multiplex editing CREEPY

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    tRNA-gRNA scaffold
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid

    #217635

    Purpose

    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

    Depositor

    Martin Kampmann

    Insert

    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha and mU6

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLO99

    Plasmid

    #90219

    Purpose

    Complements mutations in Aspergillus nidulans gene lysB (AN5206) (mutants require lysine)

    Depositor

    Berl Oakley

    Article

    Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

    Insert

    ATET_03691
    

    Use

    Aspergillus

    Tags

    Expression

    Mutation

    Promoter

    pLac

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFA6a-TurboID-3MYC-natMX6 (pFB1434)

    Plasmid

    #126050

    Purpose

    For C-terminal tagging with TurboID in yeast including nourseothricin-resistant marker

    Depositor

    François Bachand

    Article

    Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

    Insert

    TurboID
    

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPOTv7-blast-10V5

    Plasmid

    #181938

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    10xV5
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge