Addgene

We narrowed to 11,032 results for: Hal

Showing: 1681 - 1720 of 11032 results

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pERB264

Plasmid

#67764

Purpose

Tags peroxisomes with GFP and Halotag

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

PEX3-GFP-Halo

Use

Tags

GFP and Halotag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSR1038

Plasmid

#208737

Purpose

Characterization plasmid for PCap (sfgfp expressed from PCap on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PCap

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PCap

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-F5A-E389-R3A

Plasmid

#8991

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

F5A, T389E, R3A (C-terminal RSPRR motif to ASPAA)

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pGL4-23-NegCtrl

Plasmid

#163904

Purpose

Negative control vector for luciferase reporter assays

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

minimal

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pERB254

Plasmid

#67762

Purpose

Tags mitochondria with Halotag and GFP

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

Halo-GFP-Mito

Use

Tags

GFP and Halotag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pMCSG17-NleL

Plasmid

#66716

Purpose

Expresses NleL (170-782) in E. coli

Depositor

David Komander

Article

Hospenthal et al Nat Struct Mol Biol. 2013 May;20(5):555-

Insert

NleL (NEWENTRY )

Use

Tags

GST-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
p5E-QUAS (L4-R1)

Plasmid

#61374

Purpose

5' Gateway Entry Vector Containing QUAS

Depositor

Marnie Halpern

Article

Subedi et al Methods. 2014 Apr 1;66(3):433-40. doi: 1

Insert

QUAS

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-HiGlpG

Plasmid

#158538

Purpose

Bacterial expression of rhomboid protease HiGlpG

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

HiGlpG

Use

Tags

GST tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDO23A

Plasmid

#27367

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

pUC/ori, bla

Use

Gateway donor vector

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-F5A-E389-deltaCT

Plasmid

#8990

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

F5A, T389E, stop codon at amino acid 402

Promoter

Available since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pCxcl1-Firefly

Plasmid

#163896

Purpose

Luciferase reporter for mouse Cxcl1

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Cxcl1

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSpal2At

Plasmid

#78286

Purpose

Expresses A. thaliana PAL2 in E. coli

Depositor

David Nielsen

Article

McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

Insert

PAL2 (PAL2 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

*see below

Promoter

lac

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pME-QF

Plasmid

#61375

Purpose

Middle Gateway Entry Vector Containing QF

Depositor

Marnie Halpern

Article

Subedi et al Methods. 2014 Apr 1;66(3):433-40. doi: 1

Insert

QF

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR1048

Plasmid

#208738

Purpose

Characterization plasmid for PRelA (sfgfp expressed from PRelA on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PRelA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PRelA

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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CamKII alpha Venus

Plasmid

#29427

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CamKII alpha

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pSEYC68-HA-TOR2 (pAS21)

Plasmid

#39207

Depositor

Michael Hall

Article

Kunz et al J Biol Chem. 2000 Nov 24;275(47):37011-2

Insert

TOR2 (TOR2 Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

GAL

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pcSrc416

Plasmid

#17676

Depositor

David Shalloway

Article

Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Insert

c-src Y416F (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Tyr 416 changed to Phe .

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pcR295

Plasmid

#17678

Depositor

David Shalloway

Article

Bagrodia et al Nature. 1991 Jan 10. 349(6305):172-5.

Insert

c-src K295R (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Lys 295 changed to Arg.

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
Ir25a_GAL80

Plasmid

#83259

Purpose

Expresses GAL80 under the control of Ir25a enhancer

Depositor

Leslie Vosshall

Article

Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

Insert

Ir25a enhancer

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pFETCh_HHEX

Plasmid

#72353

Purpose

Donor vector for 3' FLAG tag of human HHEX

Depositor

Richard M. Myers, Eric Mendenhall

Insert

HHEX Homology Arms (HHEX Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
57F03_GAL80

Plasmid

#83254

Purpose

Expresses GAL80 under the control of 57F03 enhancer

Depositor

Leslie Vosshall

Article

Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

Insert

57F03 enhancer

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB2949

Plasmid

#8672

Depositor

David Botstein

Article

Anders et al Mol Biol Cell 2001 Dec;12(12):3973-86.

Insert

TUB1-828 under GAL1 promoter (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

D252A and E255A; dominant lethal

Promoter

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pSR1154

Plasmid

#221653

Purpose

Tetracycline sensor v3 for Staphylococcus aureus

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Inserts

sfGFP cassette
TetR cassette

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PXyl-TetO and PZwf

Available since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDestTol2-QUAS:GFP

Plasmid

#184811

Purpose

Used to generate the Tg(QUAS:GFP)c578 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-pA

Use

Zebrafish tol2 transgenesis

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR1051

Plasmid

#208739

Purpose

Characterization plasmid for PZwf (sfgfp expressed from PZwf on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PZwf

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PZwf

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR1054

Plasmid

#208740

Purpose

Characterization plasmid for PAmy (sfgfp expressed from PAmy on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PAmy

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PAmy

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR1055

Plasmid

#208741

Purpose

Characterization plasmid for PHrcA (sfgfp expressed from PHrcA on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PHrcA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PHrcA

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR1057

Plasmid

#208742

Purpose

Characterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PPgk

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PPgk

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTbp-Firefly

Plasmid

#177835

Purpose

Luciferase reporter for mouse Tbp

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Tbp

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-Chantico

Plasmid

#170659

Purpose

pUC19 vector containing an insertion of the Chantico promoter

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Chantico

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pChantico-Firefly

Plasmid

#163901

Purpose

Luciferase reporter for Chantico lncRNA

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Chantico

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-S*HiGlpG

Plasmid

#158534

Purpose

Bacterial expression of rhomboid protease mutant S*HiGlpG

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

S*HiGlpG

Use

Tags

GST tag

Expression

Bacterial

Mutation

Sector 1 and 2 mutations

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-S1*HiGlpG

Plasmid

#158535

Purpose

Bacterial expression of rhomboid protease mutant S1*HiGlpG

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

S1*HiGlpG

Use

Tags

GST tag

Expression

Bacterial

Mutation

Sector 1 mutations

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-S2*HiGlpG

Plasmid

#158536

Purpose

Bacterial expression of rhomboid protease mutant S2*HiGlpG

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

S2*HiGlpG

Use

Tags

GST tag

Expression

Bacterial

Mutation

Sector 2 mutations

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcAla12

Plasmid

#17680

Depositor

David Shalloway

Article

Yaciuk et al Mol Cell Biol. 1989 Jun . 9(6):2453-63.

Insert

c-src S12A (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Ser 12 changed to Ala.

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pcAla17

Plasmid

#17681

Depositor

David Shalloway

Article

Yaciuk et al Mol Cell Biol. 1989 Jun . 9(6):2453-63.

Insert

c-src S17A (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Ser 17 changed to Ala.

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pcSrc527

Plasmid

#17675

Depositor

David Shalloway

Article

Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Insert

c-src Y527F (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Tyr 527 changed to Phe . Activates transforming a…

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pDestTol2-QUAS:GFP-CAAX

Plasmid

#184813

Purpose

Used to generate the Tg(QUAS:GFP-CAAX)c591 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-CAAX-pA

Use

Zebrafish tol2 transgenesis

Tags

GFP-CAAX

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDESTmycPABPC4

Plasmid

#19877

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

PABPC4 (PABPC4 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4 TNRC6C

Plasmid

#20023

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Trinucleotide repeat containing 6C (TNRC6C Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge

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