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pcDNA-NBid-PhoCl2c-CBid

Plasmid

#164051

Purpose

Optogenetic manipulation of cell apoptosis using PhoCl2c

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NBid-PhoCl2c-CBid

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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LgBit-NbALFA

Plasmid

#201484

Purpose

NanoBit detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

LgBit-NbALFA

Tags

LgBit

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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TtGNN-GFP-empty

Plasmid

#106380

Purpose

dox inducible expression of GFP with neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

GFP

Use

Retroviral

Expression

Mammalian

Promoter

TRE-tight

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcpBpaRS

Plasmid

#196484

Purpose

To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

Depositor

Kensaku Sakamoto

Article

Hino et al Methods Mol Biol. 2012;794:215-28. doi:

Insert

benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA

Expression

Mammalian

Promoter

CMV (synthetase), Pol III tRNA promoter (tRNA)

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSEM272 - MCS mosTI Pmlc-2_GFP target

Plasmid

#159834

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI Pmlc-2_GFP target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-3XHA

Plasmid

#127881

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-3XHA (PPP2CA Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSEM255 - sgRNA mosTI Pmlc-2_GFP

Plasmid

#159835

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI Pmlc-2_GFP

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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TC374

Plasmid

#122614

Purpose

Prpl-28::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Expression

Worm

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP

Plasmid

#58472

Purpose

Expresses a cytoplasmic actin filament reporter, a truncation of the tandem CH domains (a.a. 1-230) from human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

Utr230 (UTRN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

Plasmid

#205278

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13B

Plasmid

#134626

Purpose

Mammalian expression of mouse KIF13B 442-1843

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF13B (Kif13b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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NbAlfa-LgBit

Plasmid

#201483

Purpose

NanoBit detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbALFA-LgBit

Tags

LgBit

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr261-EGFP

Plasmid

#58471

Purpose

Expresses a cytoplasmic actin filament reporter, the tandem CH domains (a.a. 1-261) from human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

Utr261 (UTRN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

Plasmid

#182348

Purpose

mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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1307 pcDNA3 GFP PTEN A4

Plasmid

#10760

Depositor

William Sellers

Article

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Mutation

A4: S380A, T382A, T383A, S385A

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
pMSSM16: pCMV-HA-NLS-SpuFz1

Plasmid

#205271

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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YH001 CMV-TO-tm-GS+XTEN-ADAR2(DD-E396A)-RXR (FLP-IN)

Plasmid

#233383

Purpose

ER-retained catalytically inactive ADAR2dd (E396A mutation)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

TM-ADAR2dd

Expression

Mammalian

Mutation

E396A

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140168

Purpose

Expresses a regulatable secretory cargo for Tetrahymena thermophila

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Other

Promoter

MTT1

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-FAID

Plasmid

#127885

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID (PPP2CA Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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MLSE-shREN

Plasmid

#105583

Purpose

retrovirally express control shRNA with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

control shRNA targeting luciferase

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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