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GRK4 gRNA (BRDN0001145215)

Plasmid

#77723

Purpose

3rd generation lentiviral gRNA plasmid targeting human GRK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GRK4 (GRK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GRK4 gRNA (BRDN0001146308)

Plasmid

#77724

Purpose

3rd generation lentiviral gRNA plasmid targeting human GRK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GRK4 (GRK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GRK4 gRNA (BRDN0001145617)

Plasmid

#77725

Purpose

3rd generation lentiviral gRNA plasmid targeting human GRK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GRK4 (GRK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag PKN2

Plasmid

#20587

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PKN2 (PKN2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pBabe-Puro-MEK-DD

Plasmid

#15268

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

Map2k1 (Mapk1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S218D S222D

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pLVX-Phi3-AURKB-PGK-mCherry

Plasmid

#153316

Purpose

Expresses AURKB and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

AURKB (AURKB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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MAPK1 gRNA (BRDN0001149093)

Plasmid

#77788

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK1 (MAPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK1 gRNA (BRDN0001147355)

Plasmid

#77787

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK1 (MAPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK1 gRNA (BRDN0001147498)

Plasmid

#77789

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK1 (MAPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AURKB gRNA (BRDN0001146267)

Plasmid

#77618

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AURKB (AURKB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AURKB gRNA (BRDN0001146837)

Plasmid

#77619

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AURKB (AURKB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ERK1C202

Plasmid

#164647

Purpose

Bacterial expression plasmid for His6-ERK1C202

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

ERK1C202 (MAPK1 Human)

Use

Tags

His6-tag

Expression

Bacterial

Mutation

K71R/C82S/C144S/C178S/C183S/T202C/C271S

Promoter

T7

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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AURKB gRNA (BRDN0001149202)

Plasmid

#77617

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AURKB (AURKB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ERK1C82C202

Plasmid

#164650

Purpose

Bacterial expression plasmid for His6-ERK1C82C202

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

ERK1C82C202 (MAPK1 Human)

Use

Tags

His6-tag

Expression

Bacterial

Mutation

K71R/C144S/C178S/C183S/T202C/C271S

Promoter

T7

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-NEK4

Plasmid

#23497

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK4 (NEK4 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Short isoform, only goes to aa632

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
MK01_HUMAN_D0

Plasmid

#79713

Purpose

This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Markus Seeliger, Nicholas Levinson

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK01 (MAPK1 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
Anti-Kir2.2 K+ channel [N124B/38R]

Plasmid

#140074

Purpose

Mammalian Expression Plasmid of anti-Kir2.2 K+ channel (Rat). Derived from hybridoma N124B/38.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kir2.2 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnj12 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-ADRBK1

Plasmid

#23835

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ADRBK1 (GRK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_MAPK1_WT

Plasmid

#82145

Purpose

Gateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK1 (MAPK1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-AURKB

Plasmid

#23682

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AURKB (AURKB Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge