We narrowed to 1,686 results for: pBI

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pcDNA3.1 human A3C.D99E

Plasmid

#105049

Purpose

Overexpression of APOBEC3C D99E mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Tags

HA tag

Expression

Mammalian

Mutation

Aspartate 99 to glutamate

Promoter

CMV

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.DS-EP

Plasmid

#105050

Purpose

Overexpression of APOBEC3C DS-EP mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Tags

HA tag

Expression

Mammalian

Mutation

S61P, D99E

Promoter

CMV

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.C97S

Plasmid

#105051

Purpose

Overexpression of APOBEC3C C97S mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Tags

HA tag

Expression

Mammalian

Mutation

cysteine 97 to serine

Promoter

CMV

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ166

Plasmid

#109328

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Insert

zCas9

Use

CRISPR; Gateway compatible zcas9 entry clone

Tags

3x FLAG, NLS and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAGT8163 (AATG_ttLbCas12a-i_TTCG)

Plasmid

#192453

Purpose

intronized ttLbCas12a for in planta expression

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)

Use

CRISPR and Synthetic Biology; Moclo compatible le…

Mutation

temperature-tolerant mutation (D156R)

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe-PM

Plasmid

#183168

Purpose

Construct for transient expression of plasma membrane-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

mCherry-PM

Tags

OsRac3, O. sativa rac-like GTP-binding protein 3

Expression

Plant

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGEL1029

Plasmid

#225600

Purpose

The STU backbone vector for tomato genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19/ER-mChe

Plasmid

#183163

Purpose

Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

ER-mCherry

Tags

25 aa ER signal of Glycine soja kunitz-type tryps…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19/NLS-mChe

Plasmid

#183162

Purpose

Construct for transient expression of cell nucleus-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

NLS-mCherry

Tags

16 aa nuclear localization signal and 16 aa nucl…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGEL032

Plasmid

#137901

Purpose

STU2.0 LbCas12a for plant genome editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3C-BE3

Plasmid

#113412

Purpose

Expresses hA3C-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3C-BE3 (APOBEC3C Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMDC32-FIT2

Plasmid

#96990

Purpose

Express mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Expression

Plant

Promoter

35S

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pUPD2 AtU6-26:LbCas12a_DR (GB1443)

Plasmid

#160575

Purpose

LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AtU6-26:LbCas12a_DR

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Promoter

U6-26

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGEL1024

Plasmid

#225595

Purpose

The Dual Pol II backbone vector for rice genome editing is based on Mb3Cas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL1025

Plasmid

#225596

Purpose

The STU backbone vector for rice genome editing is based on Mb3Cas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL1026

Plasmid

#225597

Purpose

The STU backbone vector for rice genome editing is based on Mb3Cas12a-R (D172R)

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL1027

Plasmid

#225598

Purpose

The STU backbone vector for rice genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL1028

Plasmid

#225599

Purpose

The STU backbone vector for maize genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)

Plasmid

#225441

Purpose

Module for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

hygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSC51

Plasmid

#104825

Purpose

CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.12g075700, Glyma.11g145900

Use

CRISPR

Expression

Plant

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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