We narrowed to 16,528 results for: puromycin

1. pY30

   Plasmid

   #84745

   Purpose

   Expresses huAsCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   puromycin resistance gene
   

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEXPqcxip-hFTSJ3-FLAG

   Plasmid

   #165463

   Purpose

   Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.

   Depositor

   Martin Dorf

   Article

   Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

   Insert

   FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3 Human)
   

   Use

   Retroviral; Gateway

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV/MSV, 5'LTR

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi-FoxO1_1R_10A_3D

   Plasmid

   #106278

   Purpose

   Fluorescent fusion protein for FoxO1

   Depositor

   Laura Heiser

   Article

   Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

   Inserts

   Foxo1a (Foxo1 Mouse)
   NLS-mCherry-NLS-P2A-Puromycin
   

   Tags

   Clover fluorescent protein

   Expression

   Mammalian

   Mutation

   Deleted amino acids 401-636, S209A, H212R, S215A,…

   Promoter

   EF1a/RPBSA

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p28-2iDP-puro

   Plasmid

   #88893

   Purpose

   Dual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.

   Depositor

   Michele Calos

   Article

   Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

   Inserts

   Dual-intron DMD platform
   luciferase
   puromycin resistance enzyme
   mCherry
   

   Expression

   Mammalian

   Mutation

   Truncated version of the dystrophin protein (tran…

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBbi-NLSmCherryNLS Clover-HDHB

   Plasmid

   #190223

   Purpose

   Fluorescent cell cycle reporter

   Depositor

   Laura Heiser

   Article

   Gross et al Nat Commun. 2023 Jun 10;14(1):3450. doi:

   Inserts

   Human DNA Helicase B (amino acids 994-1087) (HELB Human)
   NLS-mCherry-NLS-P2A-Puromycin
   

   Use

   Transposon

   Tags

   Clover fluorescent protein

   Expression

   Mammalian

   Mutation

   Amino Acids 994-1087

   Promoter

   EF1a and RPBSA

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

   Plasmid

   #110302

   Purpose

   Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

   Depositor

   Chris Kok-Lung Chan

   Article

   Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

   Insert

   p53-binding protein 1 (TP53BP1 Human)
   

   Expression

   Mammalian

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY31

   Plasmid

   #84746

   Purpose

   Expresses huLbCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICE-HA-Ku70-siR-WT

   Plasmid

   #82329

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

   Insert

   Ku70 (XRCC6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV-tet

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLCHKOv3-mCherry

   Plasmid

   #209035

   Purpose

   Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mCherry and puromycin selection marker

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PuroR-P2A-DD-TCOF1-donor

    Plasmid

    #60747

    Purpose

    Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

    Depositor

    Benhur Lee

    Article

    Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

    Insert

    TCOF1 (TCOF1 Human)
    

    Use

    Targeting donor

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCAG-Neo-PuroDTK

    Plasmid

    #84036

    Purpose

    PiggyBac cassette contains Neomycin, puromycin and TK genes

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Type

    Empty backbone

    Expression

    Bacterial and Mammalian

    Promoter

    CAG promoter

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sgGAL4-1

    Plasmid

    #46915

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgGAL4-1
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLCHKOv3

    Plasmid

    #209025

    Purpose

    Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection marker

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBbi-FoxO1_1R_13A_3D

    Plasmid

    #106279

    Purpose

    Fluorescent fusion protein for FoxO1

    Depositor

    Laura Heiser

    Article

    Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

    Inserts

    Foxo1a (Foxo1 Mouse)
    NLS-mCherry-NLS-P2A-Puromycin
    

    Tags

    Clover fluorescent protein and NLS

    Expression

    Mammalian

    Mutation

    Deleted amino acids 401-636, T24A, S209A, H212R, …

    Promoter

    EF1a/RPBSA

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNS19-LV-mutRFP-2A-GFP

    Plasmid

    #113716

    Purpose

    Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    mutRFP-2A-GFP-IRES-Puro
    

    Use

    Lentiviral; Reporter system

    Expression

    Mammalian

    Mutation

    Y64L (mutRFP)

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXPR_BRD003 sgCIC-1

    Plasmid

    #74966

    Purpose

    sgCIC-1, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    CIC (CIC Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiGuide-Puro-IFITM3g1 (BB15)

    Plasmid

    #139460

    Purpose

    Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-EF1a-CDK6-mutPAM-PGK-puro

    Plasmid

    #187638

    Purpose

    sg resistant CDK6 ORF

    Depositor

    William Kaelin

    Article

    Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    PAM site mutation

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-sgGAL4-4

    Plasmid

    #46916

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgGAL4-4
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SHIP2 WT pBABE-His-Xpress

    Plasmid

    #126584

    Purpose

    Retroviral infection of MEF cells (puromycin selection)

    Depositor

    Christophe Erneux

    Article

    Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. d

    Insert

    SHIP2 (INPPL1 Human)
    

    Use

    Retroviral

    Tags

    His Xpress (N-terminal)

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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