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pGADT7-ADH700-yeCherry-pTEF1(ashbya)-yeGFP-DHFR

Plasmid

#24585

Depositor

Thomas Wandless

Publication

Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

Insert

pADH1 yeCherry::pTEF1 (Ashbya)yeGFP-DHFR

Expression

Yeast

Mutation

1. Truncated version of ADH700 promoter (SbfI/Spe…

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only

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pRSII423

Plasmid

#35464

Depositor

Steven Haase

Publication

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast 2 micron vector

Promoter

None

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFA6a-ura4MX6

Plasmid

#49184

Purpose

gene deletion in S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Expression

Yeast

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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416Gal-FUS-YFP

Plasmid

#29593

Depositor

Aaron Gitler

Publication

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFA6a-5FLAG-bleMX6

Plasmid

#19344

Depositor

Eishi Noguchi

Publication

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

bleMX6

Expression

Yeast

Available since

Sept. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pDH3-PA-GFP

Plasmid

#18819

Depositor

Wei-Lih Lee

Publication

Vorvis et al Yeast. 2008 Sep;25(9):651-9.

Insert

Photoactivatable Green Fluorescent Protein

Use

Yeast tagging

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCAP05

Plasmid

#102920

Purpose

Direct cloning and broad-host-range heterologous expression vector

Depositor

Bradley Moore

Publication

Zhang et al MBio. 2017 Sep 5;8(5). pii: e01291-17. d

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymScarletI3-URA3

Plasmid

#224075

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection

Depositor

Bas Teusink

Insert

ymScarletI3

Tags

ymScarletI3

Expression

Yeast

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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M4755 KanMX::LEU2 Disruptor Converter

Plasmid

#51685

Purpose

Yeast marker swap plasmid for converting KanMX3 to LEU2

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LEU2 (LEU2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Publication

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Expression

Yeast

Promoter

5S rRNA-tRNA(Arg)

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pREP41-MCS+

Plasmid

#52690

Purpose

Same as pREP41, with better MCS

Depositor

Michael Nick Boddy

Type

Empty backbone

Expression

Yeast

Promoter

Pnmt41

Available since

April 30, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDR196

Plasmid

#36029

Depositor

Wolf Frommer, Dominique Loque, Doris Rentsch

Publication

Meyer et al J Biol Chem. 2006 Mar 17;281(11):7197-20

Type

Empty backbone

Expression

Yeast

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDS277

Plasmid

#34973

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

Depositor

Michael Glotzer

Publication

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

V529N

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pESC-URA-Rnq1-GFP

Plasmid

#21051

Depositor

Judith Frydman

Publication

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pFA6a-PA-GFP-TRP1

Plasmid

#18821

Depositor

Wei-Lih Lee

Publication

Vorvis et al Yeast. 2008 Sep;25(9):651-9.

Insert

Photoactivatable Green Fluorescent Protein

Use

Yeast tagging

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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pHR_A08_hrGFP

Plasmid

#84615

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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GFP-ATG8(414)/GFP-AUT7(414)

Plasmid

#49424

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

Depositor

Daniel Klionsky

Publication

Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

ATG8

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pYES2-Ub-R-GFP

Plasmid

#11953

Depositor

Nico Dantuma

Publication

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-R-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS4333 yeTadA1.0-T7 RNAP

Plasmid

#137735

Purpose

pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

Depositor

Christina Smolke

Publication

Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

Insert

yeTadA1.0-T7 RNAP

Expression

Bacterial and Yeast

Mutation

E366G (See depositor comment below)

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX-(3×FLAG)-TEV-ProtA

Plasmid

#52693

Purpose

For C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtA

Depositor

Michael Nick Boddy

Insert

(3×FLAG)-TEV-ProtA

Expression

Yeast

Available since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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