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pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

Plasmid

#87776

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Publication

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4 (MAP2K4 Human)

Tags

3xFLAG and NLS

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAav-IQGAP1-PQS1-3xFLAG

Plasmid

#84885

Purpose

rAAV-based template for genome engineering of the IQGAP1 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Publication

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

IQGAP1 genomic region HR1 (IQGAP1 Human)
IQGAP1 genomic region HR2

Use

AAV

Tags

PQS1 3xFLAG

Promoter

no

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAav-TP53-PQS1-3xFLAG

Plasmid

#84884

Purpose

rAAV-based template for genome engineering of the p53 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Publication

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

TP53 genomic region HR1 (TP53 Human)
TP53 genomic region HR2

Use

AAV

Tags

PQS1 3xFLAG

Promoter

no and no

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-3xFLAG-NLS-TPP1-OB

Plasmid

#79060

Purpose

Retroviral expression of 3xFLAG tagged, nuclear localized wild-type TPP1-OB folding domain

Depositor

Steven Artandi

Publication

Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

Insert

TPP1 (TPP1 Human)

Use

Retroviral

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Wild-type TPP-OB folding domain

Promoter

CMV

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 L555A

Plasmid

#19851

Depositor

Ron Prywes

Publication

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 L555A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 555 to Alanine

Available since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only
p414GPD-3xFLAG-Nop4 L306P

Plasmid

#169262

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 L306P

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 L306P (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

p.(Leu306Pro)

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 dE5

Plasmid

#169263

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 delta E5 (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted region corresponding to human Exon 5

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 dE8

Plasmid

#169264

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 delta E8 (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted region corresponding to human Exon 8

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 L351P

Plasmid

#169266

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 L351P

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 L351P (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

p.(Leu351Pro)

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 dE5

Plasmid

#169267

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 delta E5 (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted Exon 5

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 dE8

Plasmid

#169268

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 8

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 delta E8 (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted Exon 8

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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pWPI-Neo-LUC7L-3xFLAG

Plasmid

#165955

Purpose

Overexpression of LUC7L.

Depositor

Vamsi Mootha

Publication

Alexis A. et al Molecular Cell

Insert

LUC7L (LUC7L Human)

Use

Lentiviral

Tags

3xFLAG

Promoter

EF1-alpha

Availability

Academic Institutions and Nonprofits only

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XZ377 (PB) EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export-IRESv0-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export

Plasmid

#233385

Purpose

PiggyBac donor plasmid with optimized VEGF-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as marker

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

VEGFR1(ecto+TM)-ADAR2dd

Tags

FLAG, HA

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB410

Plasmid

#74804

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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AiP14084 - pAAV-mMbp_promoter-SYFP2-P2A-3XFLAG-10aa-H2B*-WPRE3-BGHpA (Alias: CN4084)

Plasmid

#208160

Purpose

Direct-expressing SYFP2 in oligodendrocytes AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPB CAG SMARCA4-IRES-EGFP

Plasmid

#153948

Purpose

piggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFP

Depositor

Luca Tiberi

Publication

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMS03

Plasmid

#230937

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Publication

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pOC4 cloning template vector

Plasmid

#183423

Purpose

FlpON knock-in vector with FLAG-SpCas9 expression

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDD2701

Plasmid

#175078

Purpose

Mammalian expression of Flag-tagged KSHV ORF45

Depositor

Dirk Dittmer

Publication

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8 )

Expression

Mammalian

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ku70-TC

Plasmid

#46955

Purpose

Plasmid containing the targeting construct to insert a GFP-FLAG tag at the N-terminus of the human XRCC6 gene coding for Ku70.

Depositor

Steve Jackson

Publication

Britton et al J Cell Biol. 2013 Jul 29.

Insert

XRCC6 targeting construct (XRCC6 Human)

Use

AAV; R

Tags

GFP-FLAG

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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